African Journal of
Pharmacy and Pharmacology

  • Abbreviation: Afr. J. Pharm. Pharmacol.
  • Language: English
  • ISSN: 1996-0816
  • DOI: 10.5897/AJPP
  • Start Year: 2007
  • Published Articles: 2285

Article in Press

Neutrophil-to-lymphocyte ratio of fixed dose highly active antiretroviral drugs using white rabbit

OSIME Evarista Odaburhine1* and INNIH Silvanus Olu2

  •  Received: 10 November 2015
  •  Accepted: 11 January 2016
There is an increasing reliance on neutrophil-to-lymphocyte ratio (NLR) as a prognostic marker in certain diseases. Observations have suggested the effects of administering highly active antiretroviral therapy (HAART) to human immunodeficiency virus (HIV) patients to be beneficial as well as having its side effects. Hence, this study was designed to examine the relationship of NLR of fixed dose HAART. Twenty male white rabbits weighting between 0.8 and 1.8 kg were randomly assigned to five groups A, B, C, D and E with four animals per group. Venous blood was aseptically collected before HAART administration and on days 5, 10, 15, and 20 post-treatment. Groups B, C, D, and E received graded doses of 15, 20, 25, and 34 mg/kg body weight of the drug combinations (Efavirenz, Lamivudine, and Tenofovir DF) tablets once daily for 20 consecutive days. Neutrophil-to-lymphocyte counts were determined using sysmex 4120 haematology autoanalyser. NLR was calculated by dividing the total neutrophil counted by the total lymphocyte counted. The obtained data showed a significant reduction (P<0.05) in NLR before HAART administration (controls) when compared with the administration of HAART at 15, 20, 25, and 35 mg/kg at 5 days after drug administration. The same trend was observed in the various groups at 10, 15, and 20 days after drug administration. Administration of HAART has a significant association on NLR. During HAART treatment, NLR is reduced which is a beneficial effect to the patients in the treatment of viral related infections

Keywords: Highly active antiretroviral therapy (HAART), neutrophil-to lymphocyte ratio (NLR), human immunodeficiency virus (HIV).