An efficient protocol for mass propagation of V6-2-888 sugarcane variety was optimized under this study. The shoot-tips used as an explant were surface sterilized with 0.3% (w/v) Kocide for an hour, 70% ethanol for 30 seconds and 5% Sodium hypochlorite solution for 25 minutes. Then sterilized shoot-tips were cultured on MS medium supplemented with 2mg/l BAP and 0.5mg/l IBA, 3% sucrose and 7g/l agar-agar powder. 0.5mg/l BAP and 3 mg/l Kinetin with average shoot number of 12.04 were found best for multiple shoots formation. Best root induction was observed at 2 mg/l IBA and 1mg/l NAA with 13.44 roots per shoot. All experiments were laid in Completely Randomized Design (CRD) with five replications per treatment and five explants per jar under each replicate. Data collected from each experiments were analysed using the SAS statistical software and exposed to ANOVA in conjunction with multiple comparisons (Tukey’s post hoc test). In vitro grown plantlets were then transferred to greenhouse conditions where they survived successfully. High survival rate (94.55%) was obtained in a mixture of garden soil, manure and sand at 3:1:1after four week of transplantation in glass house.