International Journal of
Biotechnology and Molecular Biology Research

  • Abbreviation: Int. J. Biotechnol. Mol. Biol. Res.
  • Language: English
  • ISSN: 2141-2154
  • DOI: 10.5897/IJBMBR
  • Start Year: 2010
  • Published Articles: 100

Full Length Research Paper

Rilpivirine and Etravirine resistance among HIV-1 infected patients failing first generation non-nucleoside reverse transcriptase inhibitors (NNRTIs) in Busia, Western Kenya

James Munyao Kingoo
  • James Munyao Kingoo
  • Department of Biochemistry and Biotechnology, School of Life and Biological Sciences, the Technical University of Kenya, Nairobi, Kenya.
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Anne WT Muigai
  • Anne WT Muigai
  • Department of Botany, School of Biological Sciences, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya.
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Viviene Matiru
  • Viviene Matiru
  • The Kenya Medical Research Institute, Centre for Virus Research. Nairobi, Kenya.
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Samoel A. Khamadi
  • Samoel A. Khamadi
  • Department of Medical Microbiology, School of Biological Sciences, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya.
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  •  Received: 02 June 2021
  •  Accepted: 15 September 2021
  •  Published: 31 October 2021

Abstract

Rilpivirine (RPV) and Etravirine (ETR) are second-generation non-nucleoside reverse transcriptase inhibitors (NNRTIs) that are not used for HIV-1 treatment in Kenya. In this cross-sectional study, we sequenced and analyzed the reverse transcriptase and pol regions of HIV-1 genome from 140 HIV infected individuals from Busia County Referral Hospital, Western Kenya, who were on anti-HIV treatment with confirmed virologic failure. All the participants were on first-generation NRTI’s and NNRTI’s for more than 12 months at the time of the study. Briefly, HIV RNA was extracted from plasma samples and sequenced to analyze for the presence of HIV-1 drug resistance mutations. The study findings showed that approximately 46% of the population had genotypic drug resistance against both Etravirine and Rilpivirine which were classified as ranging from potentially low level resistance to high level resistance despite being exposed to first-generation NNRTIs only. The study thus reveals that cross-resistance was demonstrated between primary and secondary NNRTI drugs. The development of cross resistance for RPV and ETR in patients on EFV and NVP poses a challenge in the use of these drugs as second generation NNRTI drugs.

 

Key words: Non-nucleoside reverse transcriptase inhibitors (NNRTIs), NRTIs, Kenya, cross-resistance, Etravirine (ETR), Rilpivirine (RPV), HIV-1.