International Journal of
Medicine and Medical Sciences

  • Abbreviation: Int. J. Med. Med. Sci.
  • Language: English
  • ISSN: 2006-9723
  • DOI: 10.5897/IJMMS
  • Start Year: 2009
  • Published Articles: 531

Full Length Research Paper

Experimental chronic inflammation induced in mice periodontium by Actinomyces israelii entrapped in alginate gel

Vagner Rodrigues Santos
  • Vagner Rodrigues Santos
  • Laboratory of Microbiology and Biomaterials, Facult of Dentistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.
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João Luis Duval Cândido da Silva
  • João Luis Duval Cândido da Silva
  • Laboratory of Microbiology and Biomaterials, Facult of Dentistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.
  • Google Scholar
Sérgio Monteiro Lima Jr
  • Sérgio Monteiro Lima Jr
  • Laboratory of Microbiology and Biomaterials, Facult of Dentistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.
  • Google Scholar
Felipe Alcântara Braga
  • Felipe Alcântara Braga
  • Laboratory of Microbiology and Biomaterials, Facult of Dentistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.
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Mariana Passos De Luca
  • Mariana Passos De Luca
  • Laboratory of Microbiology and Biomaterials, Facult of Dentistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.
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Vladimir Reimar A. Souza Noronha
  • Vladimir Reimar A. Souza Noronha
  • Laboratory of Microbiology and Biomaterials, Facult of Dentistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.
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Evandro Guimarães Aguiar
  • Evandro Guimarães Aguiar
  • Laboratory of Microbiology and Biomaterials, Facult of Dentistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.
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Márcio M. Mattos
  • Márcio M. Mattos
  • Laboratory of Pharmacology, Facult of Pharmacy, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.
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  •  Accepted: 17 September 2010
  •  Published: 30 October 2010

Abstract

Experimental chronic inflammation in periodontal actinomycosis was studied with INCQS standard Actinomyces israelii infected mandibula in Swiss/NIH mice (n = 45), male and female with 21 days of age. Tissue responses to experimentally induced chronic inflammation were investigated in mice by clinical and light microscopy. After anesthetization, the animals were innoculated in paraperiosteal periodontium anterior mandibula with 108 UFC/mL A. israelii (n = 45) associated with sodium alginate gel particles. The animals had been evaluated clinically and microscopicaly at 1, 3, 7, 15, 21, 30 and 45 days after innoculation. Chronic inflammation was induced in all the mice when compared to control animals. Both groups had normal general health throughout the study. In control mice (n = 10) no lesions were noted. The clinical and histopathology evolutions of actinomycosis lesions had been observed and are discuted in this work. 

Key words: Actinomycis israelii, chronic infection, alginate gel, experimental actinomycosis, periodontium, mice.