International Journal of
Medicine and Medical Sciences

  • Abbreviation: Int. J. Med. Med. Sci.
  • Language: English
  • ISSN: 2006-9723
  • DOI: 10.5897/IJMMS
  • Start Year: 2009
  • Published Articles: 531

Full Length Research Paper

Effective inhibition of agglutinability of human A and B type erythrocytes by sodium periodate-chloroacetic acid combined treatment

Dipankar Ghosh
  • Dipankar Ghosh
  • Department of Chemistry, Indian Institute of Technology, Kharagpur 721302, India.
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Susmita Santra
  • Susmita Santra
  • Department of Chemistry, Indian Institute of Technology, Kharagpur 721302, India.
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Sujit Dutta
  • Sujit Dutta
  • Hematology and Blood Bank Division, Kharagpur Sub-divisional Hospital 721302, India.
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Panchanan Pramanik
  • Panchanan Pramanik
  • Department of Chemistry, Indian Institute of Technology, Kharagpur 721302, India.
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  •  Accepted: 14 September 2009
  •  Published: 30 October 2009

Abstract

The study focused on effective inhibition of the agglutinability of human A and B type erythrocytes by sodium periodate-chloroacetic acid concerted action. Suppression of agglutinability was determined by using anti-A and anti-B antisera. Effective concentrations of sodium periodate were 6.8 and 2.7 mM, respectively for A and B type erythrocytes in presence of 4.5 mg.ml-1 chloroacetic acid in the reaction mixture. Some major bio-physiological consequences of human erythrocytes including agglutination index, spontaneous red blood cell lysis percentage, zetapotential, morphology, surface functional groups (-OH, > C = O, -COOH) and semi-quantitative oxygen uptake and phagocytic uptake were also analyzed after the chemical treatments. The future work is directed towards the universal human erythrocytes preparation from A and B type human erythrocytes.

Key words: ABH antibodies, agglutination, concerted action, erythrocyte, medicine, transfusion.