Journal of
Metabolomics and Systems Biology

  • Abbreviation: J. Metabolomics Syst. Biol.
  • Language: English
  • ISSN: 2805-4210
  • DOI: 10.5897/JMSB
  • Start Year: 2010
  • Published Articles: 10

Full Length Research Paper

Prevalence of (high-pathogenicity island) HPI-harboring Escherichia coli in diarrheic and healthy piglets

DaRong Cheng1*, XiaoLang Chen1, ShanYuan Zhu2, WenWei Ding1, ZhiXia Mu1 and Jun Zhou2, 1
1College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China. 2Jiangsu Animal Husbandry and Veterinary College, Taizhou 225300, China.
Email: [email protected]

  •  Accepted: 17 June 2010
  •  Published: 31 August 2010


One hundred and sixty four rectal swab samples were collected from 110 live diarrheic piglets and 54 healthy piglets during an investigation on the prevalence of HPI-harboring Escherichia coli (HPI + E. coli) infection associated with porcine diarrhea. The data of polymerase chain reaction (PCR) revealed the presence of HPI + E. coli in 58.18% of swabs taken from diarrheic piglets, while 44.44% of samples taken from healthy pigs were so positive. Further PCR examination of 600 bacterial isolates from diarrheic samples revealed that 25 isolates were HPI +E. coli while 4 (16%) were F4+, 2 (8%) were F4+ and F6+, 2 (8%) were F4+ and F6+, and one (4%) was F6+, LTa+ and STb+. Among the 480 bacterial isolates from non-diarrheic samples, 20 isolates were HPI + E. coli, and only one (5%) was LTa+ and STb+, while absence of isolates belonging to the F4+ and F6groups was noted. O138 was the vast prevalent serotype among the HPI +E. coli isolates. It is suggested that HPI + E. coli maybe an opportunistic pathogen in swine.


Key words: Escherichia coli, HPI, prevalence, piglet.