Abstract
Brown planthopper, Nilaparvata lugens Stål (Homoptera: Delphacidae) is one of the most threatening
pests, often significantly reducing the rice yield. The breeding of resistant cultivar has been the most
effective way of controlling this pest. Recent advancements in DNA marker technology together with
the concept of marker-assisted selection (MAS) provide new solutions for selection of more durable
brown planthopper (BPH) resistant genotypes in rice. F2s of a cross between IR 50 and CO 46 and their
susceptible and resistant parents were used for the present study. In bulk segregant analysis, random
amplification of polymorphic DNA (RAPD) primer, OPC 11 (5’AAAGCTGCGG 3’) showed co-dominant
banding pattern, which amplified a susceptible phenotype specific marker OPC11856 associated with
repulsion phase.
It also amplified resistant phenotype specific markers,
OPC 11817 which are associated
in coupling phase to the resistant allele. The OPA11817 RAPD marker could be used in a cost effective
way for marker assisted selection of BPH resistant rice genotypes.
Key words: RAPD markers, brown planthopper, bulk segregant analysis, hopper burn, Oryza sativa.
