March 2014

Laccase production by Galerina sp. and its application in dye decolorization

The laccase producing fungus HC1 was isolated from a sample collected in the Bolivian Amazon region. Analysis of 5.8S and 28S rDNA, and internal transcribed spacer 1 and 2 sequences revealed that isolate HC1 belongs to genus Galerina. High production of laccase was achieved in basal salt medium supplemented with 30 g L-1 glucose, 10 g L-1 yeast extract and 0.01 g L-1 copper sulphate. The enzyme production was further...

Author(s): Laura Mendoza, Victor Ibrahim, Maria Teresa Álvarez, Rajni Hatti-Kaul and Gashaw Mamo

March 2014

Selection of cellulase-producing Trichoderma sp. and optimization of cultivation conditions

The aim of this study was to select the wild microorganism with the capacity to produce cellulases. Microbial sources were rotten grass, leaves and straws. Natural lignocellulose as the sole carbohydrate was used to enrich microorganisms. After isolation, the strains were inoculated into liquid media. Filter paper activity (FPA) was used as an index for selection. Sequencing of 26S rDNA D1/D2 region was used to identify...

Author(s): Tian Haijiao, Qu Chang, Wu Hao, Li Yuhua and Yang Hongyan