July 2016
Ovulation induced in African catfish (Clarias gariepinus, Burchell 1822) by hormones produced in the primary culture of pituitary cells
This study examined the potential of catfish pituitary cells to proliferate in culture media and that of hormones produced in their primary culture to induce spawning in African catfish. The trypsinized pituitary cells were cultured in three culture media RPMI 1640, McCoy 5a and M2 media with addition of 10% fetal bovine serum. Female catfish (800±200 g) were induced using the culture medium that gave the...
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