June 2011

T-DNA direct repeat, vector backbone and gene trap counter selection by a new vector (pNU435) for high throughput functional genomics

The nature of T-DNA/Ds insertion decides the utility of launch pad lines for iAc/Ds based insertional mutagenesis. Direct or inverted T-DNA/Ds repeats and insertions with vector backbone lead to poor recovery of flanking sequences; whereas T-DNA/Ds insertion leading to gene trap would limit the use of such launch pad lines. A new Ds tagging/trapping vector, pNU435 containing two copies of...

Author(s): R. S. Bhat, S. S. Biradar, R. V. Patil, V. U. Patil and M. S. Kuruvinashetti

June 2011

Platelet-derived growth factor (PDGF) signaling: Detailed mechanistic insights

  It is worth mentioning that large body of experimental evidence interconnects the deregulation of specific platelet-derived growth factor (PDGF) functions to cellular transformation, yet complete picture of determinants of molecular mechanisms through which PDGF contributes to tumorigenesis remain indefinable. In this review, we have summarized current comprehension concerning PDGF functions, and...

Author(s): Ammad Ahmad Farooqi, Syed Shoaib, Ali Nawaz, Asma M Riaz, Shahzeray Mukhtar, Sehrish Minhaj, and Shahzad Bhatti