November 2010

Virological diagnosis of dengue fever in Jeddah, Saudi Arabia: Comparison between RT-PCR and virus isolation in cell culture

A total of 233 serum samples were collected from patients presenting to King Abdulaziz University Hospital with suspected cases of Dengue Fever (DF) from 2006 to 2008. Dengue virus was successfully isolated from 70 samples by culture on C6/36 and LLC-MK2 cells; it was then detected by indirect immunofluorescence assay (IFA). The cytopathic effect (CPE) of dengue virus on C6/36 appeared in most of the samples within 1-4...

Author(s): Esam Azhar, Moujahed Kao, Matthias Niedrig, Badr Masri, Azad Godus, Raed Badierah, Nassem Khan, Adnan Almazrooa, Ahmad Ashshi and Ghazi Jamjoom

November 2010

Immunogenic evaluation of a recombinant 49-kilodalton outer membrane protein of Salmonella typhi as a candidate for a subunit vaccine against typhoid

Typhoid poses a global public health concern. Presently available vaccines against typhoid have certain drawbacks that make them unsuitable for mass immunization, especially to children and elderly people. The outer membrane proteins (OMPs) of Salmonella have strong potential for the development of a candidate subunit vaccine against typhoid. We previously reported that a 49 kDa OMP confers protection against...

Author(s): Nowsheen Hamid Bhat and S. K. Jain