Use of real time polymerase chain reaction (PCR) and histopathological changes for detection of the Toxoplasma gondii parasite in male rats (experimental study)
January 2015
Toxoplasma gondii infection is wide spread in Iraq that is why this study was carried out to detect its presence in semen samples of infected male rats experimentally. Impression smears, real time polymerase chain reaction (PCR) and histopathological changes were used for detection of T. gondii. The current study included the isolation of local strains of T. gondii from placenta of women that experienced abortion that...
Isolation and identification of Mannhemia and Pasturella species from pneumonic and apparently healthy cattle and their antibiogram susceptibility pattern in Bedelle District, Western Ethiopia
December 2014
The study was conducted from October 2012 to April 2013 at Bedelle district, Western Ethiopia. The aim was to isolate Mannheimia and Pasteurella species, and to assess the associated risk factors from pneumonic and apparently healthy cattle, and antibiotic susceptibility profiles of the isolates. Out of 329 samples from animals (185 nasal swabs from clinic and 144 lung tissues abattoir) examined, 50.2% was found to be...
Production of biosurfactants by bacteria isolated from a mine tailing zone in Southern Mexico and their resistance to heavy metals
August 2014
Tailings generated through mining processes often create leachates containing high concentrations of heavy metals such as As, Fe, Mn, Zn and Pb. These high concentrations of heavy metals result in environ-mental damage such as contamination of soil, groundwater and air, which represents a huge problem for individuals living near mining areas. An alternative for soil metal removal is microbiological processes including...
Antibiogram of Escherichia coli strains isolated from food of bovine origin in selected Woredas of Tigray, Ethiopia
June 2014
Escherichia coli is a food borne pathogen causing a major public health problems. The use of antimicrobials in food animals produces resistant bacteria. To determine antimicrobial resistance of E. coli species isolated from food of bovine origin, a total of 384 of milk samples (n=192) and meat samples (n=192) were collected from different sources in 1:1 ratio in selected Woredas of Tigray, Ethiopia. Samples were...
Incidence and antibiotic susceptibility pattern of Vibrio species isolated from sea foods sold in Port-Harcourt, Nigeria
April 2014
Most Vibrio infections are associated with the consumption of raw or undercooked sea foods or exposure of wounds to warm seawater. In this study, 63 samples of a variety of sea foods viz: shrimps, periwinkle, prawn, crayfish collected from two major household markets in Port-Harcourt were cultivated using standard bacteriological method on Thiosulphate Citrate Bile Sucrose (TCBS) agar. A total of 63 Vibrios belonging to...
Detection of biofilm formation of a collection of fifty strains of Staphylococcus aureus isolated in Algeria at the University Hospital of Tlemcen
February 2014
The burden of disease caused by Staphylococcus aureus continues to grow; this organism has the ability to form biofilm and it is also a frequent cause of medical device and implant-related infections. The objective of this study was to evaluate the biofilm-forming ability of a collection of clinical isolates of S. aureus. In a total of 240 Staphylococcus spp. isolated from catheters, retrieved at five services...
Antibiotic resistance and plasmid profile of Leuconostoc spp. isolated from carrot
February 2014
In the present study, a total of 56 isolates were isolated from different root vegetables. Out of these, 17 isolates were identified as Leuconostoc spp. All the 17 isolates were checked for antibiotic sensitivity against different antibiotics. Results revealed that majority of the isolates were resistant to Penicillin G, Vancomycin, Oxacillin and Ceftazidime. Four isolates (S-9, S-13, S-37 and S-42) were resistant to...
DNA microarray-based detection of antibiotic resistance genes of human isolates of Escherichia coli in Nigeria
September 2013
Molecular epidemiology of human ecovariants of Escherichia coli from different regions of Nigeria were studied using their antibiotic susceptibility patterns, plasmid profile and DNA microarray techniques. E. coli was isolated using Eosin Methylene Blue agar (EMB) and identified by conventional microbiological technique. The isolates were tested against 14 antibiotics using the disc...
Evaluation of antimicrobial resistance of urinary tract isolated Esherichia coli from Omdurman Teaching Hospital in Sudan
September 2013
nfections caused by Escherichia coli have become a significant worldwide public health problem. The antibiotic resistance of E. coli isolated from the urine of patients of Omdurman Teaching Hospital, Sudan was investigated. The aim of this study was to evaluate the antimicrobial sensitivity of all the non serotyped urinary isolated E. coli. The antibiotic sensitivity test was carried...
Characterization of bacterial strains associated with sheath rot complex and grain discoloration of rice in North of Iran (Mazandaran province)
May 2013
In recent years, similar symptoms to sheath rot complex (SH C) and grain discoloration (GD) has been observed in paddy fields in the north of Iran. To survey the etiology of this complex disease, 207 samples of rice plants showing wide range of sheath and grain discoloration were collected during 2002-2005 at booting and ripening stages from various geographical locations in Mazandaran province....
Biodiversity of molecular profile of Staphylococcus aureus isolated from bovine mastitis cases in West Algeria
April 2013
Bovine mastitis is an inflammation of the mammary gland with local and or symptoms that occasionally result in a systemic infection. This disease has a profound impact on animal welfare and milk quality, and the most costly disease affecting dairy cows. The bacteria Staphylococcus aureus is one of the most frequently isolated pathogens from both subclinical and clinical infections. This study was...
The presence of cagA genome in blood of infected patient with Helicobacter pylori as new marker
April 2013
Helicobacter pylori is a motile and gram negative bacterium, that has been associated with gastritis, peptic ulcer, and gastric adenocarcinoma. The strains ofHelicobacter pylori contain CagA associated with severe infections. To investigate the presence of cagA gene in Helicobacter pylori strains among patients’ sera, about 100 serum specimens that had positive antibody...
Antibiotic resistance profile of Escherichia coli isolated from five major geopolitical zones of Nigeria
March 2013
Information on the resistance profiles of clinical and non clinical human bacteria isolates in the developing countries can serve as important means of understanding the human pathogens drug resistance interactions in the zone. Escherichia coliisolated from five geopolitical zones of Nigeria were screened for anti-microbial resistance profile against 14 antibiotics using the disc diffusion method....
Antibiotic resistance patterns of strains of Staphylococcus aureus isolated from patients in three hospitals in Kumasi, Ghana
March 2013
Staphylococcus aureus continues to offer challenges to medical science in the area of resistance to chemotherapeutic agents leading to treatment failures using common antibiotics. In this study, S. aureus isolated from patients from three hospitals in Kumasi, Ghana were tested for their sensitivity to some reference antibiotics using the Kirby-Bauer agar disc diffusion method. A total of...
Retrospective and clinical studies of Marek’s disease in Zaria, Nigeria
February 2013
A ten year retrospective study (2000-2009) of Marek’s disease (MD) was conducted in the Veterinary Teaching Hospital, Ahmadu Bello University, Zaria. 3,039 different poultry diseases were recorded, MD represents 4.9%. 63% of MD was recorded in birds 10 to 20 weeks old, 9.3% in birds above 30 weeks and 2.0% in birds below ten weeks. MD occurred mainly during the pre-rainy to rainy seasons with a...
Isolation and identification of a pathogen, Providencia rettgeri, in Bombyx mori
February 2013
A Gram-negative, rod-shaped bacterium, SY-1 strain, was isolated from a naturally infected silkworm Bombyx mori that was suffering from the common infection septicemia. The colony was round, slightly convex, smooth, moist, translucent, regular-edged and milky white on nutrient agar medium. The SY-1 was identified as a putative strain of Providencia rettgeri through its physiological...
Spatial and temporal variation of Escherichia coli in water and soil with relation to water parameters at the estuary of Karnafuly River, Bangladesh
January 2013
This study examined the concentrations of Escherichia coli in soil and water samples, and some physicochemical properties of water collected from the Karnafuly River estuary, Bangladesh during March-June 2012. Among the water parameters, temperature was in the range 26.5 to 32°C, salinity varied between 0 and 5 ppt, pH values ranged from 7.5 to 7.9 and dissolved oxygen (DO) was...
Effects of Mn2+ levels on the resistance properties of Bacillus cereus spores
January 2013
In some Bacillus species, manganese levels influence the resistance properties of spores. To determine if this was true for Bacillus cereus, bacteria were sporulated with different MnCl2 concentrations resulting in spores with 30-fold differences in core Mn2+ levels. Spores with different Mn2+ levels displayed no differences in resistance to dry heat, UV...
Molecular detection of Chlamydia trachomatis among gynecological patients attending Khartoum Teaching Hospital
September 2012
To perform moleular detection of Chlamydia trachomatis among gynecological patients attending Khartoum Teaching Hospital. 200 endocerical smears were collected randomly from out-patient infertile women attending the Department of Obsetrcs and Gyencology, Khartoum Teaching Hospital (Sudan). Detection of C. trachomatis was performed by the polymerase chain reaction (PCR) technique....
Characterization of antibiotic resistance in environmental enteric pathogens from Kibera slum in Nairobi-Kenya
September 2012
Kibera slum is characterized by poor sanitation hence the frequent outbreaks of diarrheal diseases. Emergence of antibiotic resistance by diarrhoea-causing bacteria is an inevitable challenge in the area. Diarrhea-causing bacteria were isolated from water, soil, vegetables, meat and dry foods samples and their antibiotic susceptibility was characterized. 237 samples were aseptically collected and...
Brucellosis: Prevalence and retrospective evaluation of risk factors in western cities of Tehran province, Iran
August 2012
Brucellosis is the commonest zoonotic disease that is spread worldwide. In this study, we aimed to compare 40 brucellosis cases with other big series in the literature in view of epidemiological and laboratory findings. A total of 40 brucellosis cases referred to Shahriar Health Center over a 3-year period were included in our study and patient files were reviewed for history and laboratory findings. Of the...
Antibiotic resistance pattern in bacterial isolates obtained from frozen food samples of animal origin in Sanandaj and Ahvaz
August 2012
One of the major public health challenges nowadays is the rapid emergence of antibiotic resistant pathogens. This study investigated the antibiotic resistance pattern in bacterial isolates obtained from frozen food samples of animal origin in Sanandaj and Ahvaz Iran. A total of 250 packages of food of animal origin were purchased from different separate grocery stores in Sanandaj and Ahvaz during one...
Community-acquired methicillin-resistant Staphylococcus aureus carrying mecA and Panton-Valentine leukocidin (PVL) genes isolated from the holy shrine in Najaf, Iraq
July 2012
Two hundred and eighty six Staphylococcus aureus isolates were collected from separate places of the holy shrine in Najaf city, Iraq. Phenotypic and genotypic examination for community associated methicillin resistant S. aureus (CA-MRSA) isolates was carried out. Antibiotic and plasmid profiles of these isolates were also done. The CA-MRSA isolates were examined using polymerase...
Bacillus megaterium, a new pathogen on lupine plants in Egypt
July 2012
The causal agents of bacterial blight in lupine (Lupinis termis) were isolated from leaves displaying symptoms in Minia governorate, Egypt. The pathogens were characterized by biochemical and physiological tests, and identified as Bacillus megaterium. Tissue extracts prepared from experimentally diseased shoot systems showed great pectolytic and cellulolytic activities while healthy tissue extracts of...
Identification and pathogenicity of phytopathogenic bacteria associated with soft rot disease of girasole tuber in Egypt
March 2012
Six bacterial isolates were isolated from naturally infected tubers of girasole plants (Helianthus tuberosus L. cv. Balady) showing soft rot, which were collected from the experimental farm of the Faculty of Agriculture, Department of Horticulture, El-Minia University, during 2010 and 2011 growing seasons. Pathogenicity tests showed the virulence of the isolated bacteria in girasole tubers...
Brevibacillus parabrevis, Acinetobacter baumannii and Pseudomonas citronellolis - Potential candidates for biodegradation of low density polyethylene (LDPE)
March 2012
Low density polyethylene is one of the polymers that is up till date nearly impossible to be degraded safely. Biodegradation is the safest method of breakdown that possibly leaves behind less toxic residue and shows potentials of bio-geo chemical cycling of the substrate. Considerable amount of work has been carried out in this area, but most of them...
A potential new isolate for the production of a thermostable extracellular α- amylase
October 2011
Two hundred and seventy Bacillus species were isolated from soil samples in Khartoum State and tested for α- amylase production. 20 potential isolates of α- amylase producer were obtained during primary screening. Secondary screening of these isolates yielded a high thermostable alkaline α- amylase producing isolate. Of all the species tested, Bacillus licheniformis gave...
Identification of single chain Fv antibody fragment against Helicobacter pylori
October 2011
Helicobacter pylori is one of the most common causes of infectious diseases around the world. Although combined antibiotic treatment for the infection exist, the development of therapies such as immunotherapy or an effective vaccine can be helpful for containment and prevention of the infection. Phage display technologies offer a simple way for selecting specific antibody fragments against a specific...
Prevalence of bacteremia in patients with diabetes mellitus in Karbala, Iraq
July 2011
The present study is designed to study bacteremia and to measure some immunological parameters of diabetic patients in Kerbala City, Iraq during the period from November 2006 until May 2007. This study included a total of 125 patients with diabetes mellitus (30 type I and 95 type II), and 55 healthy persons as Control subjects. Blood samples were collected from both patients and Controls, blood culture was...
Biochemical and molecular characterization of Haemophilus influenza isolated from Riyadh, Kingdom of Saudi Arabia
July 2011
The objective of this study focused on the prevalence of Haemophilus influenza to confirm the colonies of H. influenza on the basis of their growth requirements and serotype distribution. This study prepared 80 isolates of H. influenze isolated from five different sources (eye, ear, sputum (SP), lower genital tract (TA), and nasopharyngeal (NPA)) with different ages for infants...
Impact of Bacillus thuringiensis β – exotoxin to some biochemical aspects of Musca domestica (Diptera: Muscidae)
June 2011
The present investigation aimed to evaluate the efficiency of Bacillus thuringiensis β – exotoxin as a biological agent to control Musca domestica and to study its impact on some biochemical parameters of 2nd larval instar. Laboratory studies are carried by applying topically the bacterium B. thuringiensis β – exotoxin at LC30 under laboratory conditions (27...
In vitro susceptibility of some uropathogens and a comparative assessment of antibacterial activities of local and imported multodiscs
June 2011
The in vitro antimicrobial susceptibility of recently isolated uropathogens was investigated using two locally produced brands of antibiotics and one imported brand, in a comparative study to determine their degree of effectiveness and the susceptibility profiles of these uropathogens. Seventy eight (78) bacterial strains containing 12 different species of both Gram negative and Gram positive...
Antibacterial activity: A comparison of ripe and unripe fruit extracts of Cissus multistriata (Vitaceae) plant
May 2011
The antibacterial activity of methanol extracts of the ripe and unripe fruit of Cissus multistriata against Escherichia coli (Swine) ISB492, E. coli (Swine) ISB440, Serratia marcescens FD5/64, S. marcescens FD1/62, Staphylococcus aureus FD1/62 andBacillus cereus ISB517 were determined using agar ditch diffusion and tube dilution methods. The...
Monitoring of enteric fever and diarrhea causing bacteria in a rural setting in Nigeria
May 2011
Blood and stool samples of patients attending the General Hospital Abudu, Edo State, Nigeria were analyzed to know the prevalence of enteric fever and diarrhea causing bacteria in the area in 2006, 2007 and 2008. Blood sample was collected in Robertson cooked medium and glucose broth; then subcultured on blood agar, macConkey agar,salmonella/shigella agar and nutrient agar. Widal agglutination test was...
Colonization and antibiotic susceptibility pattern of methicillin resistance Staphylococcus aureus (MRSA) among farm animals in Saudi Arabia
April 2011
Development of Ascochyta blight [Ascochyta rabiei (Pass.) Lab.] resistant chickpea (Cicer arietinum L.) genotypes
April 2011
The present studies were conducted to estimate correlation and combining ability analysis among quantitative traits in chickpea (Cicer arietinum L.) during 2008 to 2010. Ten chickpea varieties/lines (08-AG-004, CH-70/02, CH-76/02, NOOR-91, K-70005, K-70008, K-70022, CM-2008, YN-08004 and K-70009) were evaluated for the source of resistance against Ascochyta rabiei but none was found highly...
Correlation and path coefficient studies in maize (Zea mays L.) genotypes under 40% soil moisture contents
April 2011
The present study pertaining to the estimation of genetic parameters and characters association in forty maize genotypes was conducted in the glasshouse of the Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, during the crop season in February 2009 at 40% moisture level. The data for various quantitative characters were recorded, statistically analyzed. The path-coefficients were...
Bacterial contamination: A comparison between rural and urban areas of Panipat District in Haryana (India)
March 2011
A randomized sampling from open air of the kitchens in rural vs urban households to determine bacterial contamination of Haryana (India) were carried out by taking 80 samples between July to September 2009. 40 samples of each in rural and urban area were collected in culture plates. The inoculation procedures were varied from direct inoculation of the kitchen air into the nutrient agar medium. Identification...
Biodegradation model on effect of some physicochemical parameters on aromatic compounds in fresh water medium
March 2011
Multiplex polymerase chain reaction assay for the detection of Salmonella enterica serovars in shrimps in 4 h
March 2011
A rapid and sensitive multiplex polymerase chain reaction (MPCR) based assay was developed for the detection of Salmonella enterica serovars such as Typhi (ATCC 122235), Paratyphi A (MTCC 735), Typhimurium (MTCC 98), Enteritidis (ATCC 13065), Weltevreden (MTCC 1169) Bovismorbificians (MTCC 1162), Brunei (MTCC 1168), Arizonae (MTCC 660) and Infantis (MTCC 1167) in shrimps within 4...
Influence of seedling treatment of calcium on some enzyme activity in walnut under salinity
February 2011
Levels of peroxidase (POD) and catalase (CAT) enzyme activity were determined in 2 years-old walnut plants which were grown in environmental conditions characterized by high temperatures. It’s also high photosynthetic photon flux density levels and subjected to a different salinity levels for 60 days period. The present study, through an experimental campaign, was carried out on both normally irrigated...
Induction of cinnamyl alcohol dehydrogenase in bacterial spot disease resistance of tomato
February 2011
Cinnamyl alcohol dehydrogenase (CAD) is known to be involved in the defense related responses in many host-pathogen systems. Induction of CAD enzyme in bacterial spot disease resistance in tomato (Solanum lycopersicum Mill.) was investigated in the present studies. Twenty different tomato cultivars were screened for resistance against bacterial spot disease incited by Xanthomonas...
The protective effect of beta glucan against Escherichia coli infected mice via intraperitonial administration
February 2011
Mice infected with Escherichia coli were protected against lethal peritonitis by the intra peritoneal administration 0.2 ml of 10 mg of poly-1,3,1,6-glucotriosyl-1,3,1,3-glucopyranose (PGG) glucan per gram body weight of animal 24 h prior to bacterial infection. This procedure employed in this study, is similar to that employ in a previous studies where rat model were protected with β-glucan...
Correlation of plasmid with drug resistance of clinical isolates of Escherichia coli
January 2011
According to the latest data from the Centers for Disease Control and Prevention (CDC), the six ESKAPE bacteria are responsible for two thirds of all health care-associated infections (HAIs) including Escherichia coli. Klebsiella species and their gram-negative cousin E. coli together accounted for 18 percent of all HAIs in 2006 to 2007, and a growing proportion of these two bad bugs...
Genetic variability and correlation analysis for quantitative traits in chickpea genotypes (Cicer arietinum L.)
January 2011
The present studies were conducted to the estimation of correlation for quantitative traits in chickpea (Cicer arietinum L.) in the field of the department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, during the crop season 2009 to 2010. Correlation studies showed that biomass per plant, number of pods per plant, number of secondary branches per plant, number of seeds per pod...
Comparison of in vitro activity of imipenem productions on bacterial isolates from Hashemi Nezhad Tehran hospitalized patients
October 2010
Carbapenams are one of the ß-lactamase antibiotic resistances to penicillin that were introduced in 1980 to the medical world. Imipenem- a semi-synthetic derivative of thienamycin- is one of the most important broad spectrum ß-lactamase in carbapenems. The irregular usage of drugs, especially in these types of gram-negative bacteria will lead to a serious problem in the treatment of pathogenic bacteria....
Microbiology and semen indices of sexually-active males in Benin City, Edo State, Nigeria
October 2010
This study was designed to examine the microbiological quality and semen indices of seminal fluids of sexually active males in Benin City, Nigeria, and to determine the relationship between the presence of pathogenic micro-organisms and semen parameters. Semen was collected from 229 volunteer sexually-active men, aged 19 - 33 years (mean 24.73 ± 3.4) in Benin City, Nigeria. Volunteers were advised to abstain from...
Factors influencing multi-drug resistant and plasmid DNA harbouring Aeromonas hydrophila isolated from Lake Manzala, Egypt
September 2010
Lake Manzala is considered one of the most important coastal Lakes in Egypt. This study revealed the highly polluted condition of water and fish samples taken from three of the more important sites (Kapoty, Bashtier and Mataryia areas), representative to human activity and different ecosystems in the Lake water environment. Total suspended solids (TSS), ammonia and nitrates, of the samples taken showed figures exceeding...
Antibiotics susceptibility of bacterial pathogens associated with otitis media
September 2010
One hundred twenty patients with chronic suppurative otitis media (CSOM) in Basrah, 65(54.2%) males and 55(45.8%) Females, with male: females ratio (1.2:1) and 60 individual without ontological problems as control group were included in this study, which was done during the period of March, 2009 to January, 2010. This Include collection of aural swab samples, culturing of samples, identification of causative...
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