Isolation and molecular characterization of Flavobacterium columnare strains from fish in Brazil
August 2010
Flavobacterium columnare, the etiologic agent of columnaris disease, has a broad geographical distribution and accounts for a large number of mortalities in fish species. This study aimed to generate a faster method for diagnosis of columnaris through isolation and characterization of the F. columnare 16S rDNA gene from bacteria isolated from Nile tilapia (Oreochromis...
Extended spectrum beta lactamases among multi drug resistant Escherichia coli and Klebsiella species causing urinary tract infections in Khartoum
August 2010
This is a descriptive laboratory based case study carried out in Khartoum state hospitals during the period of June, 2007 to April, 2008. The study aimed to evaluate emergence of ESBL among multi drug resistant Escherichia coli and Klebsiella species causing nosocomial UTI. Hundred strains of multi drug resistant (MDR) E. coli and Klebsiella species causing nosocomial urinary tract infections (UTIs)...
Intestinal helminth infection and anaemia during pregnancy: A community based study in Ghana
June 2010
Intestinal helminths are among the most common and widespread of human infections, contributing to poor nutritional status, anaemia and impaired growth. Anaemia and iron deficiency in pregnancy is a major public health problem in developing countries, but their causes are not always known. The objective of this study was to assess the prevalence and severity of anaemia and iron deficiencyand their association with...
Asymptomatic bacteriuria in patients with type-2 diabetes mellitus
June 2010
This study was to investigate the prevalence of asymptomatic bacteriuria (ASB) in patients clinically diagnosed with type 2 diabetes mellitus and to determine the antibiotic sensitivity pattern of bacterial isolates. One hundred and thirty type 2 diabetics comprising 56 males and 74 females (aged between 30 - 59 years) attending the Central Hospital, Benin- City, Nigeria were studied. Mid-stream urines were collected...
Some new results on affinity hemodialysis and T cell recovery
February 2010
We provide criteria under which affinity hemodialysis could provide a stable infected equilibrium. Key words: Affinity hemodialysis, HIV/AIDS envelope protein, stability criteria.
Utility of IS1245-IS1311 based PCR typing system for Mycobacterium avium isolates obtained from clinical and environmental sources
February 2010
In the present study, twenty four Mycobacterium avium isolates obtained from clinical (sputum) and environmental (water and soil) samples were analyzed by a PCR typing method. Gel electrophoresis patterns showed that a single PCR reaction generated 21 profiles. Based on the relatedness of the PCR band patterns six isolates could be grouped in three clusters. Results have the implication that the PCR based...
Conjugational transfer and survival of plasmid encoding silver and antibiotic resistance genes of Acinetobacter baumannii BL54, E. coli K12 J53.2 transconjugants and pseudomonas transformants in different soil microcosms
October 2009
In this investigation we tried to transfer plasmid encoded silver and antibiotic resistance genes from Acinetobacter baumannii BL54 to E. coli k12 J53.2 and Pseudomonas (a soil microflora) by conjugation and transformation in different soil microcosms and study the survival of the isolated bacterium in each soil. Clay loam, fine clay, sandy and clay soils were collected from different area of...
Isolation of Enterobacteriaceae and Pseudomonas spp. from raw fish sold in fish market in Khartoum state
October 2009
Enterobacteriaceae were isolated from gills, skin, muscles and the intestine of 83 out of 150 (55%) randomly collected fishes, the most dominants isolates were E. coli, Citrobacter spp, Enteriobacter spp and Klebsiella spp. This together with the highly pathogenic Enterobacteriaceae including Salmonella spp and Shigella spp.Proteus spp, and Alklegens spp....
Evaluation of in-vitro inhibitory effect of honey on some microbial isolate
September 2009
The aim of this study was to investigate the antimicrobial activity of honey sample from Basrah region against certain microbial isolate. Different concentrations (25.0, 50.0, 75.0 and 100.0%) of honey sample where checked for their antimicrobial activities, using some medically important micro-organisms includingEscherichia coli, Pseudomonas spp. and Staphylococcus aureus. The minimum inhibitory concentrations...
Structural organization of co-regulated genes in Escherichia coli
September 2009
A promoter-protein fusion library was constructed and analyzed previously which covered about 8% promoter-proximal genes from Escherichia coli in respect to their reading frames, protein productivity as well as expressional regulations under normal and various environmental stress conditions (Talukder et al., 1994, Bioscience Biochemistry and Biotechnology, 58:117-120). In this study, we further analyzed 13...
Comparison of Norwegian poultry, waterborne and clinical isolates of Campylobacter jejuni by ribotyping
August 2009
Campylobacteriosis is the most frequently occurring food and waterborne gastroenteritis in Norway and 90% of cases are caused by Campylobacter jejuni. Campylobacteriosis is epidemiologically associated with use of non-disinfected water and consumption of contaminated poultry. In order to provide molecular epidemiological support for this association isolates from local poultry and water were compared with human...
Simple method to study the mechanism of thermal and non thermal bactericidal action of microwave radiations on different bacterial species
August 2009
The present study was based on the investigations to determine whether the bactericidal effect of microwave radiations on bacteria was either thermal or due to microwaves. It was also investigated which bacterial sp is more sensitive to microwave. Five bacterial species, Proteus vulgaris PP25D, Staphylococcus aureus bgh010, Bacillu subtilis ase98, Escherchia coli TRE04 andCorynebacterium...
A microbiological technique for the separation of Hibiscus sabdariffa L. fibers
July 2009
This study is related to a process for the separation of Hibiscus sabdariffa L. fibers using living microorganisms. The selected fungal strains, Datronia sp. andOligoporous sp., were isolated from natural sources in Thailand and were previously shown to be capable of degrading lignin in pulp and paper industrial wastewater. The process described here is comprising the fungal-treated...
Amidase from plant growth promoting rhizobacterium
July 2009
Ninety three cultures of plant growth promoting rhizobacteria (PGPR) isolated from rhizosphere of Pisum sativum, among them one isolate identified asPseudomonas putida was found to be potential amidase producer. The organism exhibited a battery of PGPR traits including enhanced production of plant growth hormone indoleacetic acid (IAA) and siderophore. P. putida MTCC 6809 exhibited both intracellular...
Comparative efficacy of Rose Bengal plate test, standard tube agglutination test and Dot ELISA in immunological detection of antibodies to Brucella abortus in sera
June 2009
Comparative efficacy of three serodiagnostic tests (RBPT, STAT and Dot ELISA) in detecting anti - Brucella antibodies in sera was evaluated on a total of twenty eight serum samples which included eighteen samples from brucellosis suspected and 10 from normal healthy (brucellosis unaffected) cattle. Out of eighteen sera from suspected cases, only one (5.55%) sample was found positive by STAT and 9 (50%) samples...
Diarrheagenic Escherichia coli (DEC): Prevalence among in and ambulatory patients and susceptibility to antimicrobial chemotherapeutic agents
June 2009
The prevalence of diarrheagenic Escherichia coli both in an ambulatory patients passing out loose stools with or without blood and/or mucus in Anua General Hospital, University of Uyo Teaching Hospital and University of Uyo Health Centre from June to September, 2008 were determined using standard microbiological techniques. Susceptibility to seven different conventional and commonly available chemotherapeutic...
Calcitonin gene-related peptide and substance P significantly influence coronary flow rate in gene knockout mice
May 2009
Calcitonin gene-related peptide (CGRP) and substance P (SP) which is potent vasodilator neuropeptides play a counter-regulatory role in several models of experimental hypertension. Langendorff-perfused mouse hearts (n = 328) were used to compare coronary flow rates among wild type (WT), a-CGRP gene knockout (KO) and NK1-KO mice under various pressure loading conditions (20, 30, 40 and 50 mmHg). The aorta of each...
Skin diseases among preschool children
May 2009
In developing countries skin diseases can affect more than 60% of the general population and usually are not well managed. Skin diseases are common in children. Epidemiologic studies of the general population, however, are still limited and missing for the region of Kosova. The aim of this study was to investigate disease prevalence in children under 6 years old of out- and in-patients at the department of...
Prevalence and seasonality of fowl typhoid disease in Zaria-Kaduna State, Nigeria
April 2009
A five years retrospective study (2003 - 2007) of the prevalence of fowl typhoid (FT) and other poultry diseases diagnosed at the avian unit of the Veterinary Teaching Hospital (VTH), Ahmadu Bello University, Zaria-Kaduna State, Nigeria was conducted. A prevalence rate of 18.4% (129 cases) was recorded for FT out of 700 cases of poultry disease. The highest number of outbreaks of FT was recorded during the rainy...
Global review of meningococcal disease. A shifting etiology
April 2009
Despite expansive studies over the past century, the epidemiology of invasive meningococcal disease (IMD) has remained elusive in some of its aspects. The following review attempts to summarize the past and current trends in the etiology of IMD. Data was collected through the analysis of peer-reviewed studies and surveillance data on national, sub-national and regional levels performed using various search engines such...
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