A study on isolation and detection of drug resistance gram negative bacilli with special importance to post operative wound infection
September 2010
Isolation and identification of the drug resistant gram negative bacilli bacteria were carried out from post operative wound infections. Out of 214 samples collected, 167 showed bacterial growth and gram negative bacilli (GNB) growth was observed in 121 samples. In the present study E. coli was found to be the predominant agent isolated from wound infections (37.3%), followed...
Presence of antibiotic resistant coliforms in sachet water sold in some parts of South Eastern Nigeria
August 2010
Three hundred sachet-water samples produced in Enugu and Onitsha namely: Aqua Rapha, Bejoy, Teck Tack, SO and Rock Tama were collected from six different batches. They were screened for the presence of faecal coliforms using standard microbiology techniques. Sachet- water samples contaminated with faecal coliforms were selected and tested for their susceptibility to fourteen different antibiotics by disc diffusion...
Investigation of symbiosis between VAM fungi and root of Patchouli (Pogostemum cablin Bent.)
August 2010
Patchouli (Pogostemum cablin Bent.) is a highly valued aromatic plant. Vesicular-arbascular mycorrhizal (VAM) fungi were obtained from the roots of Pogostemon cablin. The presence of VAM improved seedling growth by facilitating the nutrient uptake of P and Zn in the P and Zn depleted soils of the controlled region of Central Institute of Medicinal and Aromatic Plants farm. VAM fungi were identified...
Comparative analysis of antibacterial activities of Xenorhabdus species on related and non-related bacteria in vivo
July 2010
Insect-nematode-bacterium mutualistic associations provide attractive systems for discovery of inter kingdom signal compounds and antibiotics. A better understanding of the biological meaning of the inter-specific diversity of compounds with antimicrobial activity of theSteinernema-symbiont Xenorhabdus bacteria may provide options for simultaneous applications in pathogen control. Anti-bacterial activities of...
In vitro activity of aminoglycosides, β lactam-β lactamases inhibitor combinations and tetracyclines against multi-drug resistant Acinetobacter baumannii, isolated from a tertiary care hospital
July 2010
Acinetobacter baumannii has emerged as a significant nosocomial pathogen, particularly in intensive care units. Isolates of A. baumannii resistant to major groups of antibiotics have been identified. These multi-drug resistant (MDR) organisms are limiting the treatment options. The study was performed to determine the in vitro activity of aminoglycosides, β lactam- β lactamase...
Antiviral activity and mode of action of Dianthus caryophyllus L. and Lupinus termes L. seed extracts against in vitro herpes simplex and hepatitis A viruses infection
May 2010
Crude Extracts of sixteen seeds belonging to different plant species were tested for their antiviral activity against herpes simplex virus-1 (HSV-1) and hepatitis A virus-27 (HAV-27). Non-toxic concentration (20 mg/ml) of Dianthus caryophyllus and Lupinus termes seedextracts to both Vero and HepG2 cells showed potent antiviral activity against HSV-1 and HAV-27 using plaque infectivity count...
HIV/vaginal candida coinfection: Risk factors in women
May 2010
To assess the association between candida and HIV infections in women from different socio-economic backgrounds, vaginal swabs were collected from 510 women (aged 9 - 83 years) attending secondary healthcare units in southern part of Benue State, Nigeria. Candida albicans was identified by microscopy. Candida infection rate was higher among HIV-infected women (88.8%, n = 116) than among HIV negative subjects...
Clinical and geographical profiles of rpoB gene mutations in Mycobacterium tuberculosis isolates from Hyderabad and Koraput in India
March 2010
Present study aims to study the geographical profile of rpoB mutations of Mycobacterium tuberculosis clinical isolates and their correlation with the therapeutic outcome. All the isolates were tested for drug susceptibility testing on Lowenstein-Jensen medium and PCR and DNA sequencing for rpoB gene mutations. Fifty two out of 101(52%) isolates were pan susceptible of which 79% were...
In vitro antibacterial activity of fronds (leaves) of some important pteridophytes
March 2010
The main objective of this research work is to screen various unexploited plants for their antimicrobial activity as these unexploited or pteridophytic plants are being used ethanomedicinally but, very little work has been done on their antimicrobial aspects. So, to explore the efficacy of these plants, the following research has been carried out. Bacterial strains of Agrobacterium tumefaciens, Escherichia coli,...
Snails as meat source: Epidemiological and nutritional perspectives
February 2010
The microbiological (epidemiological), proximate and mineral element composition of the different species of snails (Achatina fulica, Limcolaria sp. and Helix pomatia) obtained from three different market in Uyo, Akwa Ibom state were investigated. Total bacterial count ranged from 1.00 - 1.50 x 108 cfu/g, Coliform count ranged from 1.68 - 2.20 x 107 cfu/g, Salmonella/Shigella count ranged...
Characterization of surface and ground water with reference to microbiological study in Akungba-Akoko, Ondo State
February 2010
Water samples were collected from wells, bore holes and some streams at various locations within Akungba-Akoko, Ondo state. Pour plate technique was used for the estimation of Total bacterial count and Coliforms count of the water sources. Mean heterotrophic bacterial counts (cfu.ml) ranged from 1.0 x 104 in A.A.U.A storage tank to 122 x 104 in Oroke well. Total coliforms counts (cfu/ml) ranged from 0.01...
Fusidic acid-resistant methicillin-sensitive Staphylococcus aureus isolates in community of Casablanca (Morocco)
November 2009
Fusidic acid-resistant Staphylococcus aureus is poorly documented in community acquired infections in Morocco. From 1st January, 2007 - 31 October, 2008, we collected 140 S. aureus isolates at Institute Pasteur, Casablanca, 18 of them exhibited resistance to fusidic acid and were negative for gene encoding methicillin resistance (mecA). seh toxin gene was found in 14 strains (14/18) along...
Assessment of antibiotics susceptibility profiles of some selected clinical isolates from laboratories in Nigeria
November 2009
Because antimicrobial resistance patterns are continually evolving and multi-drug resistant (MDR) organisms undergo progressive antimicrobial resistance, continuously updated data on antimicrobial susceptibility profiles will continue to be essential to ensure the provision of safe and effective empiric therapies. This current study reports on the assessment of antibiotics susceptibility profiles of some selected...
Prevalence of toxigenic traits in native food isolates of Bacillus cereus in the city of Mysore, Southern India
November 2009
In the growing concern for microbial food safety of traditional foods, the present study has attempted to characterize toxigenic profile of native food isolates ofBacillus cereus. In a total of 65 traditional foods, 26 isolates were characterized by morphological, cultural and biochemical attributes as B. cereus. Of these, 12 isolates (46%) were confirmed as B. cereus by PCR...
Multiple-mutations in the katG encoding catalase proxidase in isoniazid resistant Mycobacterium tuberculosis isolates correlate with high-level of resistance in patients with active pulmonary tuberculosis in Iran
October 2009
The aim of this study was to investigate the significance of multiple-mutations in the katG gene, predominant nucleotide changes and its correlation with high level of resistance to isoniazid in Mycobacterium tuberculosis isolates that were randomly collected from sputa of 42 patients with primary and secondary active pulmonary tuberculosis from different geographic regions of Iran. Drug...
Vancomycin resistant enterococcus causing surgical site infections in a tertiary care hospital
October 2009
Presence of Vancomycin Resistant Enterococcus (VRE) is increasingly being reported. In the present study we reported the prevalence of Vancomycin Resistant Enterococcus causing Surgical Site Infections (SSI) in a tertiary care hospital. Pus from surgical site wound was cultured and Enterococcus species was identified as standard microbiological methods. The antimicrobial susceptibility for Vancomycin was determined by...
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