Effect of vitrifying solutions, ethylene glycol and 1, 2-propandiol, on intracellular K+ and lactate dehydrogenase (LDH) of hepatocytes in primary culture
Shortage of donor liver tissue for the isolation of hepatocytes requires the development of improved cryopreservation techniques for long-term storage of these cells for more efficient use. The aim of this study is to compare two cryoprotectants for preserving living cells in culture by vitrification. The viability of hepatocytes after cryopreservation was determined by the intracellular concentration of potassium...
Toxicological investigation of aqueous leaf extract of Calotropis procera (Ait.) R. Br. in Wister albino rats
The pathological changes due to the oral administration of the aqueous extract of the leaves ofCalotropis procera were studied in rats over a period of sixty days. The leaf extract was administered orally to rats in three groups receiving doses of 500, 1000 and 1500 mg/kg. The fourth group served as a control and received distilled water only. Result showed that, oral administration of the aqueous extract...