Enrichment methods for revealing lower molecular weight biomarkers in human breast plasma proteome
Breast cancer is the most common malignancy in women, comprising about 18% of all female cancers. The most important tools in screening and early detection are imaging techniques: mammography, ultrasonography and magnetic resonance imaging. However, up to 20% of new breast cancer incidents cannot be detected by these methods. Up to now, poor diagnosis of breast cancer is due to a lack of specific biomarkers....
Effect of electromagnetic field on acetylcholinesterase activity: In vitro study
The in vitro effects of extremely low frequency (ELF)-electromagnetic field (EMF) (frequencies of 50, 100, 217 Hz, induction 300 µT) on the structure and activity of acetylcholinesterase (AChE) was investigated. AChE activity was measured with UV-vis spectroscopy. Circular dichroism spectroscopy and fluorescence spectroscopy were used for investigating the secondary and tertiary structure of enzyme. The...
Matrix metalloproteinases activity in fibrosarcoma Wehi-164 cells
Fibrosarcoma is a malignant disorder of fibroblasts and extremely metastatic cancer. Angiogenesis plays an important role in metastasis. Several factors including matrix metalloproteinases (MMPs) involve in angiogenesis. MMPs are a large group of endopeptidases which degredate the extracellular matrix. The present study was conducted to evaluate the patterns of MMP-2/MMP-9 activity in Wehi-164 cells in vitro....