Recent advances in the characterization of peptidyl transferase center: Zero-distance labeling of proteins at or near the catalytic site of human 80S or Escherichia coli 70S ribosomes by means of periodate-oxidized tRNA
Periodate-oxidized tRNA (tRNAox), the 2’,3’-dialdehyde derivative of tRNA, was used as a zero-length active site-directed affinity labeling reagent, to covalently label proteins at the peptidyl transferase center (PTC), the catalytic site of the large ribosomal subunit. When human 80S or E. coli 70S ribosomes were reacted separately with tRNAox positioned at the P-site, in the...
Haematology, plasma enzymes and organ indices of Clarias gariepinus after intramuscular injection with aqueous leaves extracts of Lepidagathis alopecuroides
Tank-raised Clarias gariepinus (mean total length, 30.05±4.05cm,SD; mean weights, 250.04±50.22gSD) were injected intra-muscularly with two millilitres per kilogram of each of five concentrations (2.00, 2.00, 6.00, 8.00 and 10.00ppm) of aqueous extracts of leaves of Lepidagathis alopecuroides above the lateral line. The fish in the control group were injected with same dose of...
Levels of cadmium in different brands of cigerettes sold in Abakaliki metropolis of Nigeria
Cadmium (Cd) is recognized as category 1 carcinogen by the international agency for research on cancer based on its relationship to pulmonary tumors. Cigarette smoking is one major source of Cd in the environment. Levels of Cd in 19 different brands of cigarettes (both local and foreign made) sold in Abakaliki town were determined using atomic absorption spectrophotometer (AAS) (205). The results showed...