African Journal of
Cellular Pathology

OFFICIAL PUBLICATION OF THE SOCIETY FOR CELLULAR PATHOLOGY SCIENTISTS OF NIGERIA
  • Abbreviation: Afr. J. Cell. Path
  • Language: English
  • ISSN: 2449-0776
  • DOI: 10.5897/AJCPath
  • Start Year: 2013
  • Published Articles: 107

Full Length Research Paper

HIBISCUS-VAN GIESON STAIN FOR COLLAGEN FIBRES

Benard SA, Afolabi OO, Fowotade AA, Okoye JO, Olutunde OA, Bankole JK
Histopathology Laboratory Unit, Department of Pathology, University of Ilorin Teaching Hospital, Ilorin, Kwara State, Nigeria   Department of Pathology, College of Health Sciences, University of Ilorin, Ilorin, Kwara State, Nigeria   Medical Laboratory Science Department, School of Public and Allied, Babcock University, IlishanRemon, Ogun State, Nigeria   Department of Medical Laboratory Science, Ambrose Alli University, Ekpoma, Edo State, Nigeria
Email: [email protected]

  • Article Number - 584884369573
  • Vol.2017(9), pp. 1-4 , September 2017
  •  Received: 01 July 2017
  •  Accepted: 01 August 2017
  •  Published: 30 September 2017

Abstract

Aim: This is an experimental study aimed at exploring the capability of Hibiscus extract-iron solution counterstained with van Gieson stain to demonstrate collagen fibres in skin tissue.
Methods: 10% neutral buffered formalin fixed, paraffin embedded tissue blocks of skin were retrieved from the tissue block archive of the Department of Pathology, Unilorin Teaching Hospital, Ilorin, Kwara State, Nigeria. Two blocks were randomly selected and sectioned serially at 4 microns. The sections were primarily stained progressively with locally sourced and compounded Hibiscus sabdariffa extract-iron- solution. They were subsequently counterstained with van Gieson. Parallel sections were stained with HVG and H&E as controls.
Result: Photomicrographs reveal satisfactory staining of collagen fibers comparable to routinely used HVG.
Conclusion: This study established the capability of Hibiscus-van Gieson to demonstrate collagen fibres and could be useful in dermato-histoarchitectural studies.
 
Keywords: Hibiscus roselle, stain, van Gieson, skin, collagen

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This article is published under the terms of the Creative Commons Attribution License 4.0

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