African Journal of
Cellular Pathology

OFFICIAL PUBLICATION OF THE SOCIETY FOR CELLULAR PATHOLOGY SCIENTISTS OF NIGERIA
  • Abbreviation: Afr. J. Cell. Path
  • Language: English
  • ISSN: 2449-0776
  • DOI: 10.5897/AJCPath
  • Start Year: 2013
  • Published Articles: 107

A COMPARISON OF THE EFFECT OF COMMERCIAL pH BUFFERS ON EPITOPE RETRIEVAL OF ‘HER-2/neu’ PROTEIN

Enemari JO
  • Enemari JO
  • Department of Pathology, Ahmadu Bello University Teaching Hospital, Zaria, Nigeria.
  • Search for this author on:
  • Google Scholar
Avwioro OG
  • Avwioro OG
  • Faculty of Science, Delta State University, Abraka, Delta State, Nigeria.
  • Search for this author on:
  • Google Scholar
Osadolor HB
  • Osadolor HB
  • Department of Medical Laboratory Science, University of Benin, Benin, Nigeria.
  • Search for this author on:
  • Google Scholar
Idoko J
  • Idoko J
  • Department of Pathology, Ahmadu Bello University Teaching Hospital, Zaria, Nigeria.
  • Search for this author on:
  • Google Scholar
Yaro JD
  • Yaro JD
  • Department of Pathology, Ahmadu Bello University Teaching Hospital, Zaria, Nigeria.
  • Search for this author on:
  • Google Scholar
Odigie BE
  • Odigie BE
  • Department of Medical Laboratory Science, University of Benin, Benin, Nigeria.
  • Search for this author on:
  • Google Scholar


  • Article Number - A3FEB8B65690
  • Vol.6(1), pp. 1-5 , January 2016
  •  Received: 01 December 2015
  •  Accepted: 22 January 2016
  •  Published: 31 January 2016

References

Chazin VR, Kaleko M, Miller AD and Slamon DJ (1992). Transformation mediated by the human HER-2 gene independent of the epidermal growth factor receptor. Oncogen 7:1859 - 1866.
 

Dapson RW (2007). Macromolecular changes caused by formalin fixation and antigen retrieval. Biotech Histochem 82:133 - 140.
Crossref
 

Emoto K, Yamashita S and Okada Y (2005). Mechanisms of Heat-induced Antigen Retrieval: Does pH or Ionic Strength of the Solution Play a Role for Refolding Antigens? J. Histochem. Cytochem 53: 1311.
Crossref
 

Goldhirsch A, Coates AS, Gelber RD, Glick JH, Thurlimann B and Senn H-J. On behalf of the St Gallen Expert Panel Members. (2006). Firstselect the target: better choice of adjuvant treatments for breast cancer patients. Ann Oncol 17:1772 - 1776.
Crossref
 

Gray SD, Selbie D and Cooper RF (2007). Standardized antigen retrieval and immunohistochemical demonstration of tissue antigens using 3-in-1 buffers in conjunction with a pre-treatment module. Immunohistochemistry 6(2):58 - 61.
 

Igarashi H, Sugimura H, Maruyama K, Kitayama Y, Ohta I and Suzuki M (1994). Alteration of immunoreactivity of hydrated autoclaving, microwave treatment, simple heating of paraffin-embedded tissue sections. APMIS 102:295 - 307.
Crossref
 

Kakimoto K, Takekoshi S, Miyajima K and Osamura RY (2008). Hypothesis for the mechanism of heat-induced antigen retrieval occurring on fresh frozen sections without formalin-fixation in immunohistochemistry. J Mol Histol 39:389 - 399.
Crossref
 

Kim SH, Kook MC, Shin YK, Park SH and Song HG (2004). Evaluation of antigen retrieval buffer systems. J Mol Histol 35:409 - 416.
Crossref
 

Morgan JM, Navabi H and Jasani B (1997). Role of calcium chelation in high-temperature antigen retrieval at different pH values. J Pathol 182: 233 - 237.
Crossref
 

Penault-Llorca F, Vincent-Salomon A, Mathieu MC, Trillet-Lenoir V, Khayat D and Marty M. On behalf of the Esther Study Group. (2005). Incidence and implications of HER-2 and hormonal receptor over-expression in newly diagnosed metastatic breast cancer (MBC). J Clin Oncol 23:69.
Crossref
 

Slamon DJ, Clark GM, Wong SG, Levin WJ, Ullrich A and McGuire WL (1987). Human breast cancer: correlation of relapse and survival with amplification of the HER-2/neu oncogene. Science 235:177 - 182.
Crossref

 

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 6 No. 1
Back to articles
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES