December 2013
Identifying bacteria and studying bacterial diversity using the 16S ribosomal RNA gene-based sequencing techniques: A review
This study reflects the usefulness of sequence analysis of the 16S ribosomal RNA gene in identifying bacteria and determining bacterial diversity. Various techniques that are based on utilizing the 16S rRNA gene are discussed. Of critical importance is the use of massively parallel sequencing to study bacterial diversity. Through massively parallel sequencing which is replacing traditional methods of bacterial...
December 2013
Reduction of microbial contamination of mobile phones using ultraviolet UV radiation and ozone
Mobile phones are known to serve as habitats and breeding grounds for microorganisms, as they provide a direct contact with human body and produce the heating effect while making long calls. Microbial analysis of the University students’ and workers’ cell phones was conducted to determine total bacterial count, numbers of mesophilic aerobic bacteria and total fungal count, before and after the disinfection...
December 2013
Study of the distribution of Megasphaera micronuciformis in oral cavities of the Japanese by species-specific polymerase chain reaction (PCR) assay
Megasphaera micronuciformis is an anaerobic microbe isolated from humans. However, since the microbe is strictly anaerobic, its cultivation requires complicated facilities, making detection costly. For rapid, inexpensive detection and identification of M. micronuciformis in the clinical setting, a new technique is necessary. This study aimed to develop a species-specific PCR primer set for the detection of M....
December 2013
Biodegradation of azo and triphenylmethanes dyes: Cytotoxicity of dyes, slime production and enzymatic activities of Staphylococcus epidermidis isolated from industrial wastewater
In view of compliance with increasingly stringent environmental legislation imposed by regional, national and supranational (for example, European Union) authorities, innovative environmental technologies for the treatment of dye-contaminated effluents are necessary in the color industry. In this study, azo (methyl orange (MO) and methyl red (MR)) and triphenylmethanes (malachite green (MG), fushin (F) and crystal...
December 2013
Evaluation of biofilm formation of Klebsiella pneumoniae isolated from medical devices at the University Hospital of Tlemcen, Algeria
Isolates of Klebsiella pneumoniae are responsible for opportunistic infections in humans, particularly of the urinary respiratory tracts. These bacteria express type 3 fimbriae that have been implicated in binding to eukaryotic cells and matrix proteins. Twenty four (24) K. pneumoniae strains isolated from medical devices were studied. Their capacity to form biofilm was assessed using two types of materials,...
December 2013
Antimicrobial and anticancer activities of endophytic fungi from Mitrajyna javanica Koord and Val
A study was conducted for screening the antimicrobial and anticancer activities of endophytic fungi isolated from leaves of Mitragyna javanica Koord and Val. A total of 73 fungal endophytes were obtained. Fifty-five isolates (75.47%) showed antimicrobial activities against at least one tested microorganism by the paper disc diffusion method. Out of these isolates, 5.47% displayed a broad antimicrobial spectrum. The...
December 2013
Isolation of a new Staphylococcus epidermidis strain with anti-mycobacterial activity and determination of the nature of its active substance
Tuberculosis is the second cause of death from infectious disease. The resurgence of tuberculosis and the increase in mycobacterial infections, as well as the multidrug-resistance of these bacteria to available antibiotics, has encouraged research on new antimycobacterial agents. In this context, water and soil samples from different Moroccan biotopes have led to the isolation of a bacterial strain, capable of...
December 2013
Identification of Herpes virus in patients with chronic periodontitis from Northeastern Mexico
Periodontitis is an inflammatory process that occurs in the tissues surrounding the teeth. It is caused by microorganisms and groups of microorganisms and results in loss of periodontal ligament and alveolar bone. Herpes virus has emerged as a putative pathogen in periodontal disease. Periodontal disease was demonstrated in 50 patients by careful probing of pockets and completion of a medical record. Three saliva...
December 2013
Isolation and characterization of glyphosate-degrading bacteria from different soils of Algeria
Glyphosate (N-phosphonomethylglycine) is the most commonly used herbicide worldwide. Due to the concern regarding its toxicity for non-targeted species in soil, finding glyphosate-degrading microorganisms in soil is of interest. The success of this will depend on isolating bacteria with the ability to grow in presence of glyphosate. Five bacterial strains were isolated from different untreated soils of Algeria, the...
December 2013
Real-time polymerase chain reaction (PCR) detection of Trichomonas vaginalis from urine samples of human immunodeficiency virus (HIV) and acquired immunodeficiency syndrome (AIDS) patients in Limpopo Province, South Africa
Trichomonas vaginalis is a protozoan parasite that infects the human urogenital tract. This study determined the prevalence and risk factors of T. vaginalis infection amongst human immunodeficiency virus (HIV) infected patients in Limpopo Province using urine-based real time polymerase chain reaction (RT-PCR). Urine samples were collected from 155 patients attending three public hospitals and one private clinic in the...
December 2013
Evaluation of total fungal air contamination levels and efficiency of the ventilation systems used in adult haematology unit and adult stem cell transplantation unit
This study is aimed to measure the fungal loads in the protected areas with different ventilation systems, including the stem cell transplantation unit and haematology sevice to compare the results with the outdoor air. Using volumetric air samplinc method, fungal load was investigated in the air; samples were taken from 11 points for a period of 25 weeks. Concentrations of fungal conidium were identified by the...
December 2013
The role of hematites (bloodstones) communally used in barbershops in the transmission of Hepatitis B virus
In many countries, an apparatus called hematite (bloodstone) is used to stop bleeding caused by shaving in men in barbershops. One hematite may be used for more than one person. Till date, there are no studies that demonstrate the role of these stones in hepatitis B virus (HBV) transmission. The aim of this study was to show the role of hematites used for more than one person in barbershops in the transmission of...
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