Discrimination of Ralstonia solanacearum isolates by genetic signatures produced by single-strand conformation polymorphism and low-stringency single specific primer PCR analysis
Development of molecular biology based techniques have led to reliable characterization and determination of the genetic diversity among phytopathogens. Single-strand conformation polymorphism (SSCP) and Low-stringency single specific primer (LSSP)-PCR were assessed for genetic typing of Ralstonia solanacearum isolates from suspicious bacterial wilt fields. R. solanacearum isolates obtained were amplified by colony PCR...
Lactic acid bacteria from traditionally processed corn beer and palm wine against selected food-borne pathogens isolated in south west region of Cameroon
The present study was undertaken to assess the inhibitory potential of lactic acid bacteria isolated from traditionally processed corn-beer and palm-wine on Escherichia coli, Salmonella typhi and Staphylococcus aureus. Lactic acid bacteria were isolated on MRS agar using pour plate method. The catalase negative and Gram positive isolates were selected as presumptive lactic acid bacteria and were biochemically...
Detection of microbial surface contamination and antibiotic resistant Escherichia coli on beef carcasses in Arusha, Tanzania
Food serves as an important fomite for transmission of disease-causing and antibiotic resistant bacteria to humans. Because this may be an especially challenging problem in low-income countries, the level of microbial surface contamination and abundance of antibiotic resistant Escherichia coli on beef carcasses were estimated. Out of 125 surface swab samples (plated on MacConkey agar), 70 to 100% were positive to...
Characterization of Fusarium oxysporum isolates from tomato plants in Algeria
Fusarium oxysporum is an ubiquitous soil-borne fungus, having a high genetic and ecological diversity with the potential to cause diseases of many crop species of economic interest. Indeed, some strains of F. oxysporum known as pathogens generate common diseases such as wilting, root and crown rot on host plants. Two formae speciales are confined to the tomato: F. oxysporum f.sp. lycopersici (FOL) causing Fusarium wilt,...
Growth inhibition of the stored fish (Ethmalosa fimbriata) fungus Aspergillus flavus, exposed to extracted essential oils from Callistemon citrinus and Ocimum canum
The aim of this study was to evaluate the antifungal effects of essential oils from Callistemon citrinus and Ocimum canum against Aspergillus flavus. Major components in the oil of C. citrinus were 1,8-cineole (60.6%), α-pinene (18.5%), limonene (5.0%) and α-terpineol (5.0%). The oil of O. canum was mainly composed of 1.8-cineole (20.8%), linalol (14.3%), eugenol (11.9%), terpinen-4-ol (7.4%) and germacrene...
Characterization of Bacillus cereus spores isolated from Algerian processed cheese
The main purpose of this study is to characterize the isolated Bacillus cereus spores from processed cheese manufactured and marketed in Algeria. Sixty samples of four brands of processed cheese were analyzed. The panC gene sequencing identified four B. cereus spores belonging to Group III, according to the classification of Guinebretiere et al. (2010). In nutrient broth, values â€‹â€‹of D for B. cereus spores...