African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Table of Content: 14 January, 2015; 9(2)

January 2015

Physico-chemical surface characterization of Bacillus cereus spores isolated from an Algerian dairy plant

Bacillus cereus is an endospore-forming bacterium frequently found in dairy products and dairy environment. In this study, the hydrophobicity and surface electrical charge of spores from fourteen (14) Bacillus cereus strains isolated from a dairy plant located in north-western Algeria (were studied using microbial adhesion to hydrocarbon (MATH) method, and zeta potential measurements, respectively. Spores of eleven (11)...

Author(s): DIDOUH Nassima, CHÉRIF-ANTAR Asma, BENAMAR Ibrahim and MOUSSA-BOUDJEMÂA Boumedine

January 2015

Prevalence and distribution of methicillin resistant Staphylococcus aureus (MRSA) among laboratory science students and laboratory staff from a single hospital in North Saudi Arabia

Staphylococcus aureus causes morbidity and mortality in both community and hospital settings. Methicillin resistant S. aureus (MRSA) is being increasingly reported globally. This study aimed at finding out the prevalence and the distribution of the community-associated MRSA (CA-MRSA) or healthcare-associated MRSA (HA-MRSA) among 150 subjects. Of the 150 subjects, 125 were students of laboratory science and 25 were...

Author(s): Mohammed Suliman Al-Mogbel

January 2015

Characterization and identification of lactic acid bacteria isolated from traditional cheese (Klila) prepared from cow's milk

Various types of fermented dairy products exist worldwide. Their nature depends on the type of milk used, pretreatment, fermentation conditions and subsequent treatment. The fermentation of milk primarily involves lactic acid bacteria (LAB). Among these, the Klila is a hard variety cheese made by using the traditional procedures in the home, without using a starter culture. The different samples of traditional cheese...

Author(s): Guetouache, M., and Guessas, B.

January 2015

β-Lactam - β-lactamase inhibitor combinations as the choice therapy for multidrug resistant Acinetobacter

Acinetobacter, an important nosocomial pathogen, is capable of causing infectious outbreaks in critically ill patients which results into high morbidity and mortality worldwide. It is rated among top seven pathogens that disturb the health care delivery system. The situation has become complicated due to the organism’s capability to acquire diverse resistance mechanisms. This has resulted in the emergence of...

Author(s): Farhan Rasheed, Raja Kamran Afzal and Muhammad Jan Leghari

January 2015

Identification of volatile compounds, antimicrobial properties and antioxidant activity from leaves, cones and stems of Cupressus sempervirens from Algeria

Cupressus sempervirens L. (Cupressaceae) leaves, cones and young branches have been used in traditional medicine and aromatherapy. The composition of the isolates obtained by hydrodistillation from the aerial parts (plant material collected at Bainam forest, in Northwest of Alger, Algiers), were analyzed by GC and GC-MS. The leaves isolate (yield 0.22% w/w), was mainly composed of monoterpene hydrocarbons (60.8%),...

Author(s): Nafila Zouaghi, Cherifa Bellel, Carlos Cavaleiro, Boubekeur Nadjemi and Mohamed Yousfi

January 2015

Obtaining DNA from Staphylococcus aureus: A study on DNA extraction methods for food matrices without bacterial isolation

Polymerase chain reaction (PCR) is a tool widely used. However, for the effective use of PCR, DNA quality is necessary. Thereby, the objective of this study was to evaluate DNA extraction methods of Staphylococcus aureus, for direct application in food. Five methods were tested using reference and food strains. The methods show different characteristics with steps of thermal lysis, enzymatic lysis, detergents and...

Author(s): Marjory Xavier Rodrigues, Gabriela Sartori Felkl, Renata Louize Samulak, Andiara Gonçalves Tenório and Juliana Vitória Messias Bittencourt

January 2015

Induction of defense-related proteins and growth promotion in tomato by mixture of Trichoderma harzianum OTPB3 and Bacillus subtilis OTPB1 and Pseudomonas putida OPf1 against Phytophthora infestans

Late blight incited by Phytophthora infestans is a destructive disease of tomato worldwide. The plant growth-promoting antagonists, which elicit induced systemic resistance (ISR) and enhance plant growth, are being used as safe alternatives to synthetic fungicides for the management of plant diseases. In this study, a combination of Trichoderma harzianum OTPB3 and Bacillus subtilis OTPB1 and Pseudomonas putida OPf1...

Author(s): S. P. Mohan Kumar, P. Chowdappa, V. Krishna and H. Sandhya

January 2015

Microbiological assessment of traditional smoked silver catfish (Chrysichthys nigrodigitatus)

Smoked fish is relished food item in many dishes in Nigeria. Traditional smoked silver catfish (Chrysichthys nigrodigitatus) that floods the smoked fish market of the Lagos State of Nigeria are not microbiologically shelf-stable; hence, the need for a study on their microbiological quality and safety. ‘Fresh silver catfish’ (100 samples) and smoked silver catfish (100 samples) were collected from 20...

Author(s): Adeyeye, S. A. O., Oyewole, O. B., Obadina, A. O. and Omemu, A. M.

January 2015

Inducing salinity tolerance in chickpea (Cicer arietinum L.) by inoculation of 1-aminocyclopropane-1-carboxylic acid deaminase-containing Mesorhizobium strains

Salinity stress severely affects growth, nodulation and yield of chickpea (Cicer arietinum L.). However, inoculation with Mesorhizobium strains containing 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase improves the plant growth by reducing the level of ethylene induced by salt stress. Fifty (50) Mesorhizobium isolates were obtained from nodules of chickpea plants on yeast extract mannitol agar (YEMA) medium....

Author(s): Deepika Chaudhary and Satyavir Singh Sindhu

January 2015

Effects of gelatin coating glass coverslips on fungal attachment and their morphological demonstrations

Gelatin obtained from collagen, an extracellular matrix (ECM) protein, plays a crucial role in facilitating cell attachment. This study aimed to determine the effects of gelatin on fungal attachment and morphology. Samples of Aspergillus niger were grown on gelatin-coated coverslips and non-gelatin-coated coverslips, and prepared for examination under both light and scanning electron microscopes. The results show...

Author(s): Supattra Suwanmanee and Natthanej Luplertlop

January 2015

Antimicrobial activity of extracts from an endemic Salvia cilicica Boiss. and Kotschy

The antimicrobial activities of the different aerial and root extracts of Salvia cilicica were determined aiming to evaluate whether, it can be used in phytotherapy as an antimicrobial agent. In this study, the antimicrobial activity of roots and aerial parts of S. cilicica extracts was evaluated using micro dilution and disc diffusion methods against Gram positive and Gram negative reference standard microorganisms and...

Author(s): Nur Tan, Burcu Sen, Merve Bilgin and Emir Tan

January 2015

Detection of Leptospira interrogans in plasma and urine samples by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)

Acute phase of leptospirosis has high mortality. A diagnosis can be designed with high sensitivity by testing blood and urine samples by polymerase chain reaction (PCR) for early diagnosis of this phase of disease. The aim of this research was to detect Leptospira interrogans by PCR technique in plasma and urine samples which were taken from symptomatic patients. Clinical isolates were obtained in hospitals of north...

Author(s): Ramazan Rajabnia, Elaheh Ferdosi Shahandashti, Somayeh Yaslianifard, Elham Ghadami, Asefe Kheirandish and Nafiseh Adhami