May 2016

Incidence of zoonotic Campylobacter jejuni in fast meal meat, grill chickens and symptomatic Egyptians

Campylobacter jejuni is one of the most important foodborne gastroenteric zoonosis. Most strains of C. jejuni produce a toxin (cytolethal distending toxin) that hinders the cells from dividing and produces diffuse bloody edematous exudative enteritis. The common routes of transmission are fecal-oral, person-to-person and the eating of raw or undercooked chickens or meat. This study recognizes the incidence of zoonotic...

Author(s): Hassan A. El Fadaly, Ashraf M. A. Barakat, Khaled A. Abd El-Razik, Azza S. M. Abuelnaga and Elgabry A. Elgabry

May 2016

Efficacy of plasmid curing agent on Streptomyces longsporesflavns

Plasmid-specified traits like lactose metabolism and L-asparginase production could be eliminated from Streptomyces longsporesflavns culture during production of fermented product (Microbial succession). In this study we mainly focus on plasmid profiling and plasmid characterization from S. longsporesflavns isolated from marine soil. Then we observe the efficacy of plasmid curing agent on S. longsporesflavns....

Author(s): T. R. Sivashankari, K. Sudha, S. Barathi and V. Karthikeyan

May 2016

Suppression of duck hepatitis A virus Type 1 replication by lentivirus-mediated RNA-dependent RNA polymerase (RdRp) gene-specific siRNA

To explore the application of RNAi technology for the control of duck hepatitis A virus type 1 (DHAV-1). The RNA-dependent RNA polymerase (RdRp) gene was amplified by PCR. pEGFP-RdRp was constructed for fusion expression of EGFP-RdRp protein. According to the sequence of RdRp, three gene-specific siRNAs were designed and the corresponding shRNA was inserted into pmiRZipΔ to construct pRdRp-shRNA. pRdRp-shRNA and...

Author(s): Yong-Juan Wang, Shan-Yuan Zhu, Ping-Fu Cui, An-ping Wang, Wei-Ming Hong, Hui Lu and Wei-Yong Zuo

May 2016

Nodular bacterial endophyte diversity associated with native Acacia spp. in desert region of Algeria

Five species of Acacia (Acacia ehrenbergiana Hayne, A. nilotica (L.) Delile, A. seyal Delile, A. tortilis (Forssk.) Hayne and A. laeta Delile) indigenous to Tamanrasset (Algeria) were investigated for their nodulation status and nodular endophytic diversity. A. ehrenbergiana showed the highest nodulation ability across the different sites in this region, indicating the widespread occurrence of compatible rhizobia in the...

Author(s): Zineb Faiza Boukhatem, Chahinez Merabet, Abdelkader Bekki, Sonia Sekkour, Odile Domergue, Robin Dupponois and Antoine Galiana