African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Table of Content: 16 December, 2011; 5(30)

December 2011

Reduced infectivity and environmental resistance of microsporidian Nosema bombycis after spore wall proteins-removal treatment

  Nosema bombycis is a highly virulent causative agent of the pebrine disease in sericulture. The only infective stage of the parasite is the environmentally resistant spore, on which the protein-rich spore wall supposedly contributes to their infection and resistance. In this study, we explore the potential roles of spore wall proteins (SWPs) of mature N. bombycis spores by evaluation of...

Author(s): Zeng Tu, Qiangbo Liu and Yongji Wan

December 2011

New mutations existing in the nucleocapsid (N) gene of non-porcine TGEV strains isolated in China

  Transmissible gastroenteritis virus (TGEV) is the etiological agent of TGE, and non-porcine hosts are potential carriers of TGEVs. In this study, genomic RNA were extracted from TGEVs designated HYM-09, HYM-09-1 and HYM-09-2 isolated respectively from non-porcine hosts (dogs, cats and foxes) naturally infected with TGEVs. The nucleocapsid (N)genes of the three...

Author(s): Chaolai Man and Xiaolong Yu

December 2011

Aerobic treatment of lipid-rich wastewater by a bacterial consortium

  Lipids raise handicap in the treatment of wastewaters in the purification stations. For that, we studied the aerobic biological treatment of greases of synthetic wastewater and restaurant wastewater by selected and optimized bacteria consortium. The results of this work highlighted the beneficial effect of the adaptation of bacteria which was evaluated by the study of chemical oxygen demand (COD) abatement...

Author(s): Abdelali Fadile, Fatima Zahra El Hassani, Halah Aissam, Mohammed Merzouki and Mohamed Benlemlih

December 2011

Multilocus sequence analysis of the Rhizobia from five woody legumes in Southern China

  A total of 11 rhizobial strains from five woody legumes (Acacia confusa, Dalbergia odorifera, Dalbergia fusca, Erythrophleum fordii and Pterocarpus macarocarpus) in Southern China were identified, using 16S rRNA gene sequence analysis and multilocus sequence analysis (MLSA) of three housekeeping genes (recA, glnII and dnaK). Among the 11 isolated rhizobia, seven...

Author(s): Junkun Lu, Lihua Kang, Xinhua He and Daping Xu

December 2011

Elevated antibody titer against Borrelia burgdorferi in new case of multiple sclerosis patients

  Multiple sclerosis (MS) is a chronic, inflammatory, demyelinating disease that affects central nervous system (CNS). The etiology of MS is unknown, but it likely involves a combination of genetic, non-genetic and environmental factors such as microbial agents. In this descriptive case- control study, 61 new cases of MS patients and 60 healthy controls were studied for IgM and IgG antibody titer...

Author(s):   Mohsen Khaki, Ehsanollah Ghaznavi-Rad, Ali Ghazavi, Keyvan Ghasami and Ghasem Mosayeb

December 2011

Antibacterial activity of quercetin on oral infectious pathogens

  The aim of this study was to evaluate the antimicrobial activity of quercetin against pathogens of the main oral infectious diseases which include caries, periodontitis and oral mucosa infectious diseases associated microorganisms. Agar diffusion assay was adopted to observe the effects of quercetin on the growth of 11 main oral pathogenic microorganisms, including Streptococcus mutans, Streptococcus...

Author(s): Yi Shu, Yi Liu, Li Li, Jin Feng, Beiyan Lou, Xuedong Zhou and Hongkun Wu

December 2011

Study of a lead tolerant yeast strain BUSCY1 (MTCC9315)

  The present study highlights isolation, characterization and bio-adsorption strategy of a lead tolerant yeast strain designated BUSCY1 from industrial effluent of Chittaranjan Locomotive Workshop, Burdwan, West Bengal, India. The isolate was found to grow best on yeast extract peptone sucrose (YEPS) medium (2% sucrose and 0.2% yeast extract found to be optimum for growth) with profuse production of extra...

Author(s): Sabyasachi Chatterjee, Debdoot Gupta, Pranab Roy, Narayan Chandra Chatterjee, Pradipta Saha and Sikha Dutta

December 2011

Effectiveness of inoculation with isolated Anoxybacillus sp. MGA110 on municipal solid waste composting process

  Anoxybacillus sp. MGA110, a thermophilic cellulolytic bacterium, was isolated from municipal solid waste (MSW) composting and characterized by 16S rRNA sequencing. A pure culture of the isolated strain was applied to municipal solid waste composting as inoculum. The results of inoculation showed that the biodegradation of organic matter was quicker than the control as indicated by more reduction in C/N...

Author(s):   Shabnam Ghaffari, Abbas Akhavan Sepahi, Mohammad Reza Razavi, Fereydon Malekzadeh and Hossein Haydarian

December 2011

Antimicrobial activity of the aqueous extract of selected Malaysian herbs

  Malaysia is rich in biodiversity encompassing a variety of herbs and shrubs with potential medicinal properties. Tinospira crispa (Patawali), Anacardium occidentale (Gajus),Garcinia atroviridis (Asam gelugor) and Hibiscus cannabinus (Kenaf) are some of the well-known local Malay herbs that have been studied for their medicinal applications. The objective of the present study...

Author(s): Z. A. Zakaria, M. L. Zakaria, Z. Amom and M. N. M. Desa

December 2011

The emergence of plasmid mediated quinolone resistance genes qnrA, qnrB, and qnrS among Egyptian clinical isolates of Enterobacteriaceae

  Quinolone resistance is emerging in Gram-negative pathogens worldwide. In the present study, the prevalence of this problem was determined for different species ofEnterobacteriaceae that were isolated from clinical specimens mainly from Ain Shams University Hospitals at Cairo, Egypt. Escherichia coli had the largest number of isolates, and relatively showed the highest resistance...

Author(s): El-Essawy A. K., Abu Shady H.M. and El-Biaa B. M.

December 2011

Prevalence of Listeria spp. in fish obtained from Urmia fish markets

  The present study was conducted to determine the prevalence of Listeria spp. in fish obtained from Urmia fish markets. A number of 194 fish comprising Oncorhynchus mykiss (n=42), Sander lucioperca (n=38), Cyprinus carpio (n=30), Hypophthalmiachthys molitrix (n=48), Abramis brama (n=12), Astacus leptodactylus (n=12) and Silurus...

Author(s): Rojan Modaresi, Karim Mardani, Amir Tukmechi and Abdolghaffar Ownagh

December 2011

Epidemiological and clinical characteristics of gastroenteritis associated with enteric adenovirus in hospitalized children in Bushehr Province, Iran

  Enteric adenoviruses are one of the most significant etiological agents of severe viral gastroenteritis among infants and young children worldwide. This study was conducted to evaluate the clinical and epidemiological features of enteric adenovirus gastroenteritis in hospitalized children less than 7 years of age in Borazjan, Bushehr Province, Iran. From October 2008 to September 2010,...

Author(s): Akram Najafi, Hassan Shafiei, Afshin Barazesh, Shariat Najafi, Mah-Khanom Mohammadian, Katayoun Vahdat and Mohammad Kargar

December 2011

Molecular characterization of fowl adenoviruses associated with hydro-pericardium syndrome in broilers

  Hydropericardium syndrome (HPS) is a viral disease of poultry, which is common in hot and humid season during summer in Pakistan. Fowl adenovirus serotype 4 (FAdV-4) classified into the species Fowl adenovirus C and the genus Aviadenovirus, which were identified as the causative agent of the disease during field outbreaks.  The virus was obtained from suspected broiler birds showing typical signs...

Author(s): Shah, M. S., ,Ashraf, A., Khan, M. I., Rahman, M., Habib, M., Babapoor, S., Ghaffar, A., Malik, I. R., Khannum, S. A. and  Qureshi, J. A.,

December 2011

Bacterial contamination of healthcare workers' mobile phones and efficacy of surface decolonization techniques

  This study was performed to determine the rate of bacterial contamination of mobile phones of healthcare workers and the efficacy of 70% Ethyl alcohol and 70% Isopropyl alcohol as disinfectant agents. 150 mobile phones of healthcare workers in Esfahan's hospital were included. Samples were collected by sterile, moistened swabs and were cultured on blood agar and EMB and then isolates were identified. In...

Author(s): Maryam Mohammadi-Sichani and Vajiheh Karbasizadeh

December 2011

Evaluation of the dry sheet medium (Compact Dry LS) for screening of total Listeria count in food samples

  The Compact Dry LS (CD-LS), ready-to-use dry sheet selective medium for Listeriaspecies, was evaluated for inclusivity and exclusivity by using 107 strains including 20Listeria species strains. All tested Listeria species strains other than Listeria seeligeri that grew as blue colored colony on CD-LS. CD-LS were compared with those on Oxford agar and ALOA agar as conventional...

Author(s): Hajime Teramura, Masafumi Uchida, Mitsuaki Kashida and Hidemasa Kodaka

December 2011

Ultra-structure of the yeast Trichosporn mycotoxinivorans and its effect on some biological parameters in mice

  In this research work, the ultra-structure of yeast, Trichosporon mycotoxinivorans was studied by scanning and transmission electron microscopy. Effects of yeast on biological parameters in mice including body weight, mortality rate and compounds in blood (glucose, total protein, cholesterol, and insulin) were estimated. Blood constituents (White blood cells (WBC), Red blood cells (RBC) haemoglobin...

Author(s): Abdulla S. Khaliel, Jamal M. Khaled and Saleh A. Kandeal,

December 2011

Detection of Norwalk virus in marine foods in Iran

  The objective of this study was to determine the prevalence of Norwalk virus in marine foods samples in Isfahan and Shahrekord townships of Iran. From September 2010 to April 2011, a total of 300 samples of fresh fish, shrimp, crab and lobster samples were obtained from randomly selected retail stores in Isfahan and Shahrekord townships of Iran. The samples were tested for the presence...

Author(s): Hassan Momtaz Ebrahim Rahimi Rahil Farzan

December 2011

Interplay between calcium influx and nitrate assimilation in Spirulina platensis

  Nitrate assimilation and its interplay with Ca2+ ion transport has been studied in Spirulina platensis PCC 7345, an alkaliphilic filamentous cyanobacterium. We have usedverapamil, a calcium-channel blocker and sodium orthovanadate, a calcium efflux blocker, to study the effect of nitrate assimilatory setup. Nitrate uptake (NU) increased significantly with verapamil but was...

Author(s): Pradeep Kumar Logeswaran, Vinky Kohli and B Vani

December 2011

Comparison of pathogen enrichment methods for detecting Acidovorax avenae subsp. citrulli” from watermelon seeds

  It is well known that bacterial fruit blotch (BFB) of watermelon caused by Acidovorax avenae subsp. citrulli threatens watermelon production. There is no pathogen enrichment or concentrated steps before detection in conventional PCR-based diagnostic technique, which consequently leads to the lower detection sensitivity. In the present study, two different bacterial suspensions were...

Author(s): WANG Jing, LIU Qing, CHEN Nian-lai, LIU Jin-zhi, and BI Yang

December 2011

The frequency of slime, adhesin and methicillin resistance genes among staphylococci isolated from nasal samples of multiple sclerosis patients

  It was aimed to determine the carriage rate of Staphylococcus aureus and the occurrence of methicillin resistance, slime and adhesin genes in staphylococcal strains isolated from the nasal cavities of multiple sclerosis (MS) patients. The presence of mecA and femA, and the genes implicated in adhesion were determined by multiplex PCR in all strains. The femA gene was...

Author(s): Ismet M. Melek, Nizami Duran, Gülay G. Duran, TaÅŸkın Duman and Esra Okuyucu

December 2011

Analysis on 72 cases of adverse reactions of Chinese medicine injection

  To provide doctors and patients with the guidelines for reasonable use of Chinese medicine injection through the analysis on the adverse reactions of Chinese medicine injection in use. Statistics and analysis are made on the raw data of 72 patients that is age, allergic history, ADR manifestations and prognosis. It is found that the adverse reactions of Chinese medicine involve organs and systems. They are...

Author(s): Zhongjun Zhang, Guanglei Wang and Gongjian Liu

December 2011

Synergistic antibacterial effects of nano zinc oxide combined with silver nanocrystales

  Antibacterial nanocrystals have attracted great interests in recent years. In fact, with the emergence and increase of microbial organisms resistant to multiple antibiotics, many researchers have tried to develop new antibiotics. The aim of this research is to compare antibacterial activity of mono-metallic with composite nanocrystals, againstEscherichia coli, Salmonella galinarium, Staphylococcus...

Author(s): Alireza Jafari, Masood Ghane and Shahrdad Arastoo

December 2011

Investigation and analysis of the related knowledge, attitude and behavior on AIDS of the FSWs in Jiangsu Province, China

  To understand the status of related knowledge, attitude, the practical situation of behavior on AIDS of the female sex workers (FSWs) in the places of entertainment in Jiangsu Province in order to provide a more effective scientific evidence for prevention and control the prevalence of AIDS. The 867 entertainment FSWs in three cities in Jiangsu province were interviewed by the trained interviewers using...

Author(s): Wenzhe Ma, Yang Wen, Minghui Zhang, Xiping Huan, Haitao Yang, Zhihang Peng, Rongbin Yu, Lu Wang, Ning Wang, Hongbing Shen, and Feng Chen

December 2011

Arabidopsis WRKY28 transcription factor is required for resistance to necrotrophic pathogen, Botrytis cinerea

  Arabidopsis thaliana WRKY28, which is strongly induced by H2O2 stress, is one of the group II WRKY proteins and responds to Botrytis cinerea. In this study, we successfully generated up and down-regulating WRKY28 transgenic A. thaliana lines. Our investigationinto the action of WRKY28 demonstrated that the down-regulation of WRKY28 resulted in susceptible...

Author(s): Lin-tao Wu, Gui-mai Zhong, Jian-mei Wang, Xu-feng Li, Xu Song and Yi Yang

December 2011

Detection of Clostridium tetani by fluorescent amplification based specific hybridization

  The etiologic agent of tetanus, Clostridium tetani, is an obligate anaerobic and spore-forming bacillus; and remains an important public health problem in developing countries. Although, the diagnosis is based mainly on history and presentation of tetanus symptoms. Some recent reports indicate that a patient may not manifest any symptoms of localized or generalized tetanus. However, one of...

Author(s):   Mohammad Saadatmandzadeh, Hossein Eshghi , Mohammad Rahimizadeh, Mojtaba Sankian and Saeid Amel Jamehdar

December 2011

Isolation of proteolytic, lipolytic, and bioemulsifying bacteria for improvement of the aerobic treatment of poultry processing wastewater

  Proteolysis, lipolysis, as well as, emulsification play important roles for the biological treatment of poultry processing wastewater. Three isolates identified by 16S rRNA gene sequence analysis as belonging to Bacillus subtilis, Bacillus thuringiensis, andLysinibacillus fusiformis, were then selected. The three combined strains provided a 71.2± 0.20% reduction of the chemical...

Author(s): Rattana Tarntip and Thungkao Sirichom

December 2011

Molecular detection of methicillin resistance gene (mec A gene) and pathogenic genes among Staphylococcus aureus isolates from clinical and drinking water samples of HIV and AIDS patients in Limpopo Province, South Africa

  In the present study, Staphylococcus aureus strains isolated from de-identified clinical samples including urine, sputum, and stools obtained from Human Immunodeficiency virus (HIV) and Acquired immunodeficiency syndrome (AIDS) patients as well as their drinking water samples were analysed. Antibiotic resistance was tested by the disc diffusion while biofilm was tested using the microplate method....

Author(s): Samie A. and Shivambu N.

December 2011

Sulphonylureic herbicidal risk in the detection of soil fungi communities

  Cambic chernozem soil samples were collected from experimental field of plant breeding discipline, the U.S.A.M.V.B. department in TimiÅŸoara. The soil has been sifted through a sieve of 2 mm and placed in polyethylene bags. Thereafter, the soil was treated with two herbicides (tribenuron-methyl and nicosulfuron) in different doses. Samples were incubated for 7 days in a thermostat at 28°C...

Author(s): Marioara Nicoleta Filimon, Aurica Breica Borozan, Despina Maria Bordean, Roxana Popescu, Smaranda Rodica Gotia, Doina Verdes and Sinitean Adrian

December 2011

Prevalence of drug resistant and exotoxin A producing Pseudomonas aeruginosa in cutaneous infections in a tribal area in South India

  Pseudomonas aeruginosa is one of the most important clinically significant and opportunistic pathogens, often causing nosocomial infections. Pseudomonas has been implicated to cause folliculitis and other papular or vesicular lesions in the skin of otherwise healthy individuals besides pyoderma gangrenosum in neutropenic patients. In this study, Pseudomonas species were isolated...

Author(s): Neha Ghosh, A. K. Goel, Harivadan Lukka, P. Bhattacharya and D. V. Kamboj

December 2011

Determination of some metabolites of Cordyceps sobolifera

  Cordyceps sobolifera is an edible Chinese native medicinal material. Profiles of mannitol, polysaccharides, total saponins and total flavonoids in 12 populations of C. soboliferawere analyzed to evaluate the characteristics of this species. The amount of mannitol was uniformly distributed among the populations, which ranged from 5.78 to 10.91 g/100 g. The highest point reached by polysaccharide was...

Author(s): H. G. Liu, T. Li, Y. L. Zhao, J. Zhang and Y. Z. Wang

December 2011

Study of trichodinids (Protozoa, Ciliophora) parasitic on gills of freshwater fishes from Chongqing, China, and identification of a new species Trichodina cyprinocola sp. nov.

  This study surveyed freshwater fishes Cyprinus carpio and Carassius auratus from Chongqing, China, for infection with ectoparasitic trichodinids. Fish specimens were obtained from culture pools, smears of gills were prepared and used for silver nitrate impregnation. Microbiologic studies identified two species of trichodinids belonging toTrichodina Ehrenberg, 1838 including one new...

Author(s): Fahui Tang and Yuanjun Zhao

December 2011

Antimicrobial resistance pattern and prevalence of metallo-β-lactamases in Pseudomonas aeruginosa from Saudi Arabia

  To determine the prevalence of metallo-β-lactamases (MBLs) in Pseudomonas aeruginosa and to investigate whether MBL genes have spread in MBL-producing isolates. A total of 350 clinical isolates of P. aeruginosa were screened for production of MBL. Antibiotic susceptibility testing was determined by E-test strips. Six MBL genes and class 1 integron were tested by polymerase chain...

Author(s): Mohamed H. Al-Agamy, Atef M. Shibl, Samar A. Zaki and Abdulkader F. Tawfik