July 2014
Biological nitrogen fixation by diazotrophic bacteria in association with grasses of economic importance
The capacity to transform atmospheric N2 into ammonia, which initially enhanced the research, has become just one of the potentialities verified by diazotrophic bacteria, and today, it is known that these microorganisms produce various plant-growth-promoting substances. Due to the enormous data obtained so far, diazotrophic bacteria have the potential to be used as biofertilizers in different crops or grasses, it is...
July 2014
Suppression of Fusarium wilt disease of banana by Zimmu (Allium cepa L. x Allium sativum L.) leaf extract
Among 33 botanicals screened against Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Fusarium wilt disease of banana, only the Zimmu leaf extract recorded 100% inhibition of both mycelial growth and spore germination of the Foc pathogen. Further evaluation of Zimmu leaf extract at different concentrations under greenhouse conditions in cv. Grand Naine (Cavendish-AAA) showed complete suppression of Fusarium...
July 2014
Effect of ultraviolet-B (UV-B) radiation on bacterial community in the soybean phyllosphere
The impact of ultraviolet-B (UV-B) radiation on the culturable and unculturable bacterial communities were studied in field experiments using soybean plants grown under increased UV-B (UV-B+), negative control (UV-B-) and solar UV-B. Sampling of leaves exposed to UV-B was performed in different developmental stages of the plant. The data obtained demonstrate that UV-B radiation did not alter the culturable and...
July 2014
Simulation and characterization of UV reactor for wastewater treatment
Several mathematical models are developed to explain microorganism responses to ultraviolet irradiation. During this study, Pseudomonas aeruginosa was taken as a microorganism model. The experimental results obtained during a batch UV-reactor established that the disinfection kinetics is far from being uniform over the batch. Application of the first-order Chick-Watson model, in its original form or modified form, to...
July 2014
Development of a real-time PCR assay for the detection and quantification of Gluconacetobacter diazotrophicus in sugarcane grown under field conditions
The objective of this study was to evaluate the viability of real-time polymerase chain reaction (PCR) to detect Gluconacetobacter diazotrophicus in sugarcane inoculated and non-inoculated with diazotrophs which are grown under field conditions. The primer pair PAL5F and PAL5R yielded a specific band of 189 bp using real time PCR with SYBER Green I. This primer pair was the most sensitive one to detect...
July 2014
Antibacterial resistance pattern of aerobic bacteria isolated from patients with diabetic foot ulcers in Egypt
The purpose of this study was to determine and evaluate the current status of antimicrobial resistance, both phenotypically and genotypically, of the most common aerobic pathogenic bacteria recovered from diabetic foot ulcers (DFUs) in Egypt. Standard methods were used for culture identification, sensitivity testing and extended spectrum β-lactamases (ESBLs) detection. PCR for bla genes was performed and the...
July 2014
Anthraquinones and triterpenoids from Senna siamea (Fabaceae) Lam inhibit poliovirus activity
The methanol extract of stem bark of Senna siamea (Fabaceae) was found to contain compounds with anti-poliovirus activity. Bioassay-guided fractionation of the extract resulted in the isolation and identification of six compounds; three triterpenoids- lupenone (1) lupeol (4) and betulinic acid (5); two antraquinones- chrysophanol (2) and physcion (3); and β- sitosterol glucoside (6). To evaluate the capacity of the...
July 2014
Staphylococcus aureus in soup samples from restaurants/food canteens and some families in Jalingo Metropolis, North-Eastern Nigeria
A prospective study was conducted in 2011 and 2012 to determine the occurrence and antibiotic resistant pattern of Staphylococcus aureus in soups prepared and sold in restaurants/food canteens and some selected families in Jalingo Metropolis, Taraba State, Nigeria. Two hundred (200) soup samples comprising of 100 from restaurants/food canteens and 100 from selected families were collected and processed. Out of the 200...
July 2014
Isolation and characterization of phosphate solubilizing bacteria (Klebsiella oxytoca) with enhanced tolerant to environmental stress
The present investigation was aimed at the isolation and characterization of phosphate solubilizing bacteria with enhanced tolerant to environmental stress. The bacterial strain isolated from a metal-contaminated soil collected from abandoned mines was identified as Klebsiella oxytoca according to 16S rRNA analysis. The strain proved the ability to solubilize inorganic phosphate under a wide range of pH (4 to 10),...
July 2014
Antiparasitic effects of Allium paradoxum as a conventional consumed vegetable
Giardiasis is an important common intestinal infection; caused by the protozoan parasite Giardia lamblia. Numerous medications are available for the treatment of Giardiasis and can be prescribed by the health care provider. Metronidazole and other chemical drugs are used for Giardiasis. Considering the side effects of these drugs like other chemical agents, this prompted us to analysis an in vitro research into...
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