Abstract

A reverse flow injection chemiluminescence (CL) method for the fast and simple determination of vitamin C has been developed. The CL light observed after luminal-H₂O₂ aqueous solution was added to the on-line formed Br₂ solution from mixing of HBr and KBrO₃ solutions was inhibited in the presence of Vitamin C. Based on these facts, a determination method of Vitamin c was developed. In order to obtain the optimum experimental conditions, effects of different variables on the chemiluminescence intensity and proposed flow-injection analysis (FIA) system were investigated. The calibration curve obtained under optimum conditions was linear over the range from 1.0 to 16.0 µg/ml with correlation coefficient R = 0.9959 and detection limit of 0.4 µg/ml. The proposed method has been successfully applied to the determination of Vitamin C content in pharmaceutical formulation. The obtained results are in good agreement with an iodimetric method reported in British Pharmacopeia.

Key words: Reverse-FIA, chemiluminescence, vitamin C, pharmaceutical formulation.

Abbreviation

Abbreviations: CL, Chemiluminescence; AA, ascorbic acid; FIA, flow-injection analysis;DDW, deionized-distilled water; RSD, relative standard deviation.