Abstract

The antioxidant capacity of doum fruit extract and the total phenolic content were analyzed. The antioxidant capacity was estimated by DPPH and iron chelating assays. Quercetin, ascorbic, BHT and tannic were used as positive controls. Also the effect of doum extract on viability of acute myeloid leukemia was studied. The results showed that the total phenolic content were 0.5 µg/ 3 µg dried extract sample as quercetin. In iron chelating assay the result showed that 800 µg/ml doum extract gave the best antioxidant activity (21% inhibition). In DPPH assay the 1000 µg/ml extract exhibited 50% antioxidant activity (IC₅₀) but 1500 µg/ml extract exhibited 80% antioxidant activity. In the viability test, the results showed that half maximal inhibitory concentration (IC₅₀) of doum extract was found to be 3 µg/ml. The result indicated that the doum extract could be an important dietary source of phenolic compounds with high antioxidant and anticancer activities.

Key words: Hyphaene thebaica, total phenols, antioxidant and anticancer activities, AML.