African Journal of
Pure and Applied Chemistry

  • Abbreviation: Afr. J. Pure Appl. Chem.
  • Language: English
  • ISSN: 1996-0840
  • DOI: 10.5897/AJPAC
  • Start Year: 2007
  • Published Articles: 368

Full Length Research Paper

Major anthocyanin quantification, free radical scavenging properties and structural identification in Cymbopogon giganteus extracts

Remy K. Bationo
  • Remy K. Bationo
  • Laboratoire de Chimie Organique et Physique Appliquées (L.C.O.P.A.), Université Joseph KI-ZERBO, UFR/SEA 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Constantin M. Dabire
  • Constantin M. Dabire
  • Laboratoire de Chimie Organique et Physique Appliquées (L.C.O.P.A.), Université Joseph KI-ZERBO, UFR/SEA 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Yougoubo Abdoulaye
  • Yougoubo Abdoulaye
  • Laboratoire de Chimie Organique et Physique Appliquées (L.C.O.P.A.), Université Joseph KI-ZERBO, UFR/SEA 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Boukare Kabore
  • Boukare Kabore
  • Laboratoire de Chimie Organique et Physique Appliquées (L.C.O.P.A.), Université Joseph KI-ZERBO, UFR/SEA 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Ousseni Sawadogo
  • Ousseni Sawadogo
  • Laboratoire de Chimie Organique et Physique Appliquées (L.C.O.P.A.), Université Joseph KI-ZERBO, UFR/SEA 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Moumouni Koala
  • Moumouni Koala
  • Laboratoire de Chimie Organique et Physique Appliquées (L.C.O.P.A.), Université Joseph KI-ZERBO, UFR/SEA 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Eloi Pale
  • Eloi Pale
  • Laboratoire de Chimie Organique et Physique Appliquées (L.C.O.P.A.), Université Joseph KI-ZERBO, UFR/SEA 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Roger H. Ch. Nebie
  • Roger H. Ch. Nebie
  • CNRST/IRSAT/ Département Substances Naturelles, 03 BP 7047 Ouagadougou 03, Burkina Faso.
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  •  Received: 26 May 2023
  •  Accepted: 28 July 2023
  •  Published: 31 August 2023

Abstract

Anthocyanin-based colorants are highly appreciated by the food industry for their lower toxicity. However, in Burkina Faso, very little scientific information is available on potential sources of anthocyanins, despite significant progress in plant studies. This scientific report is the first to focus on anthocyanins of Cymbopogon giganteus from Burkina Faso and aims to valorize them for their use as an alternative to synthetic food colorants. The method using 2,2-diphenyl-1-picryhydrazyl (DPPH) reagent, the differential pH test, and Liquid Chromatography and High Performance Thin Layer Chromatography (LC and HPTLC) coupled to ultraviolet and mass spectrometry were used, respectively to determine free radical scavenging properties, anthocyanin content, and structure of anthocyanin compounds. Anthocyanin content evaluation showed values between 0.109 and 1.974 mg cyanidin equivalent per gram of extract. The free radical scavenging properties ranged from 305 to 446 µg/mL and we were able to identify in the high-content anthocyanin extracts, sixteen (16) anthocyanins compounds.

Key words: Anthocyanins compounds, molecular characterization, mass spectrometry, LC-UV-MS/MS, free-radical properties.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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