Abstract

A novel and selective method for the fast determination of trace amounts of Pb(II) ions in liver loggerhead turtles specimens has been developed. A reliable and rapid method for preconcentration and modification of organic-solution-processable functionalized-nano graphene with Isopropyl 2-[(isopropoxy carbothioyl) disulfanyl] ethanethioate (IIDE) in order to prepare an effective sorbent for the preconcentration and determination of lead. The sorption capacity of modified organic-solution-processable functionalized-nano graphene (IIDE MS) was 82.34 mg.g^-1 and the optimum pH for the quantitative recovery of lead was found as 5.3. The optimum flow rate, sorbent amount and sample volume were 8 ml.min^-1, 300 mg and 50 ml, respectively. 10 ml of 0.1 mol.L^-1 HCl was the most suitable eluent. The recommended method is simple and reliable for the determination of lead without any notable matrix effect and successfully applied to environmental water samples. The limit of detection of the proposed method is 7.5 ng/ml. The method was applied to the extraction and recovery of Pb(II) in different water samples. In the present study, we report the application of preconcentration techniques still continues increasingly for trace metal determinations by flame atomic absorption spectrometry (FAAS) for quantification of lead in Formalin-fixed paraffin-embedded (FFPE) tissues from liver loggerhead turtles. This method exhibits the superiority in compared to the other adsorption reagents because of the fact that there is no necessity of any complexing reagent and optimum pH of solution presents in acidic media. In this method, the relative standard deviation (RSD) of 2.7%.

Key words: Organic-solution-processable functionalized-nano grapheme, lead, Isopropyl 2-[(isopropoxy  carbothioyl)disulfanyl] ethanethioate (IIDE) -modified silica-gel, formalin-fixed paraffin-embedded (FFPE), tissues from liver loggerhead turtles.