Continuous extraction of bitter kola nuts, followed by liquid-liquid extraction and flash column chromatography leads to the isolation of garcinoic acid (ï¤-tocotrienoloic acid), as the main lipophilic component in a 0.8% yield of the initial dry weight. This compound, an oxidised member of the vitamin E family, was structurally characterised using nuclear magnetic resonance spectroscopy.
Key words: Vitamin E, tocopherol, antioxidant, two-dimensional nuclear magnetic resonance spectroscopy.
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