Biotechnology and Molecular Biology Reviews

  • Abbreviation: Biotechnol. Mol. Biol. Rev.
  • Language: English
  • ISSN: 1538-2273
  • DOI: 10.5897/BMBR
  • Start Year: 2006
  • Published Articles: 103

Review

DNA microarrays and their applications in medical microbiology

Chijioke A. Nsofor
  • Chijioke A. Nsofor
  • Department of Biotechnology, Federal University of Technology, Owerri, Imo State, Nigeria.
  • Google Scholar


  •  Accepted: 02 November 2013
  •  Published: 28 February 2014

References

Akondi KB, Lakshmi VV (2013). Emerging Trends in Genomic Approaches for Microbial Bioprospecting. J. Integr. Biol. 17(2):61-70.
 
Anjum MF, Mafura M, Slickers P, Ballmer K, Kuhnert P, Woodward MJ, Ehricht R (2007). Pathotyping Escherichia coli by using miniaturized DNA microarrays. Appl. Environ. Microbiol. 73:5692-5697.
Crossref
 
Anthony RM, TJ Brown, French GL (2000). Rapid diagnosis of bacteremia by universal amplification of 23S ribosomal DNA followed by hybridization to an oligonucleotide array. J. Clin. Microbiol. 38:781-788.
Pubmed
 
Antonova OV, Gryadunov DA, Lapa SA, Kuz'min AV, Larionova EE, Smirnova TG, Nosova EY, Skotnikova OI, Chernousova LN, Moroz AM, Zasedatelev AS, Mikhailovich VM (2008). Detection of mutations in Mycobacterium tuberculosis genome determining resistance to fluoroquinolones by hybridization on biological microchips. Bull. Exp. Biol. Med. 145:108-113
Crossref
 
Aragon LM, Navarro F, Heiser V, Garrigo M, Espanol M, Coll P (2006). Rapid detection of specific gene mutations associated with isoniazid or rifampicin resistance in Mycobacterium tuberculosis clinical isolates using non-fluorescent low-density DNA microarrays. J. Antimicrob. Chemother. 57:825-831.
Crossref
 
Ballmer K, Korczak BM, Kuhnert P, Slickers P, Ehricht R, Hachler H (2007). Fast DNA serotyping of Escherichia coli by use of an oligonucleotide microarray. J. Clin. Microbiol. 45:370-379.
Crossref
 
Battaglia C, Salani G, Consolandi C, Bernardi LR, De Bellis G (2000). Analysis of DNA microarrays by non-destructive fluorescent staining using SYBR green II. BioTechniques 29:78-81.
Pubmed
 
Behr MA, Wilson MA, Gill WP, Salamon H, Schoolnik GK, Rane S, Small PM (1999). Small,Comparative genomics of BCG vaccines by whole-genome DNA microarray. Science 284:1520-1523.
Crossref
 
Bøving MK, Pedersen LN, Moller JK (2009). Eight-plex PCR and liquid-array detection of bacterial and viral pathogens in cerebrospinal fluid from patients with suspected meningitis. J. Clin. Microbiol. 47:908-913
Crossref
 
Call DR, Borucki MK, Loge FJ (2001b). Detection of bacterial pathogens in environmental samples using DNA microarrays. J. Microbiol. Methods. 53: 235-243.
Crossref
 
Call DR, Bakko MK, Krug MJ, Roberts MC (2003). Identifying antimicrobial resistance genes with DNA microarrays. Antimicrob. Agents Chemother. 47:3290-3295.
Crossref
 
Call DR, Chandler DP, Brockman F (2001a). Fabrication of DNA microarrays using unmodified oligonucleotide probes. BioTechniques 30:368-379.
Pubmed
 
Call DR, Borucki MK, FJ Loge (2003). Detection of bacterial pathogens in environmental samples using DNA microarrays. J. Microbiol. Methods 53:235-243.
Crossref
 
Cassone M, Marco MD, Francesco I, Marco RO, Gian MR and GP (2006). DNA Microarray for Detection of Macrolides Resistance Genes. Antimicrob. Agents Chemother. 50(6):2038-2041
Crossref
 
Chizhikov V, Wagner M, Ivshina A, Hoshino Y, Kapikian AZ, Chumakov K (2002). Detection and genotyping of human group A rotaviruses by oligonucleotide microarray hybridization. J. Clin. Microbiol. 40:2398-2407
Crossref
 
Cleven BE, Palka-Santini M, Gielen J, Meembor S, Kronke M, Krut O (2006). Identification and characterization of bacterial pathogens causing bloodstream infections by DNA microarray. J. Clin. Microbiol. 44:2389-2397.
Crossref
 
Crameri A, Marfurt J, Mugittu K, Maire N, Regos A, Coppee JY, Sismeiro O, Burki R, Huber E, Laubscher D, Puijalon O, Genton B, Felger I, Beck HP (2007). Rapid microarray-based method for monitoring of all currently known single-nucleotide polymorphisms associated with parasite resistance to antimalaria drugs. J. Clin. Microbiol. 45:3685-3691.
Crossref
 
Deng J, Zheng Y, Zhao R, Wright PF, Stratton CW, YW Tang. (2009). Culture versus polymerase chain reaction for the etiologic diagnosis of community-acquired pneumonia in antibiotic-pretreated pediatric patients. Pediatr. Infect. Dis. J. 28:53-55.
Crossref
 
Diaz MR, Fell JW (2005). Use of a suspension array for rapid identification of the varieties and genotypes of the Cryptococcus neoformans species complex. J. Clin. Microbiol. 43:3662-3672
Crossref
 
Fitzgerald C, Collins M, van Duyne S, Mikoleit M, Brown T, Fields P (2007). Multiplex,bead-based suspension array for molecular determination of common Salmonella serogroups. J. Clin. Microbiol. 45:3323-3334.
Crossref
 
Fukushima M, Kakinuma K, Hayashi H, Nagai H, Ito K, Kawaguchi R (2003). Detection and identification of Mycobacterium species isolates by DNA microarray. J. Clin. Microbiol. 41:2605-2615
Crossref
 
Gegia M, Mdivani N, Mendes RE, Li H, Akhalaia M, Han J, Khechinashvili G, Tang YW (2008). Prevalence of and molecular basis for tuberculosis drug resistance in the Republic of Georgia: validation of a QIAplex system for detection of drug resistance-related mutations. Antimicrob. Agents Chemother. 52:725-729
Crossref
 
Gentry TJ, Zhou J (2006). Microarray-based microbial identification and characterization. In: Y. W. Tang and C. W. Stratton (ed.), Advanced techniques in diagnostic microbiology. Springer Science and Business Media, New York, NY. pp. 276-290.
Crossref
 
Hager J (2006). Making and using spotted DNA microarrays in an academic core laboratory. Methods Enzymol. 410:135-168.
Crossref
 
Hakenbeck R, Balmelle N, Weber B, Gardes C, Keck W, de Saizieu A (2001). Mosaic genes and mosaic chromosomes: intra- and interspecies genomic variation of Streptococcus pneumoniae. Infect. Immun. 69:2477-2486.
Crossref
 
Hegde P, Qi R, Abernathy K, Gay C, Dharap S, Gaspard R, Hughes JE, Snesrud E, Lee N, Quackenbush J (2000). A concise guide to cDNA microarray analysis. BioTechniques 29:548-562.
Pubmed
 
Honma S, Chizhikov V, Santos N, Tatsumi M, Timenetsky MD, Linhares AC, Mascarenhas JD, Ushijima H, Armah GE, Gentsch JR, Hoshino Y (2007). Development and validation of DNA microarray for genotyping group A rotavirus VP4 (P[4], P[6], P[8], P[9], and P[14]) and VP7 (G1 to G6, G8 to G10, and G12) genes. J. Clin. Microbiol. 45:2641-2648
Crossref
 
Hsiao CR, Huang L, Bouchara J-P, Barton R, Li HC, Chang TC (2005). Identification of medically important molds by an oligonucleotide array. J. Clin. Microbiol. 43:3760-3768.
Crossref
 
Huang A, Li J-W, Shen Z-Q, Wang X-W, M Jin (2006). High-throughput identification of clinical pathogenic fungi by hybridization to an oligonucleotide microarray. J. Clin. Microbiol. 44:3299-3305.
Crossref
 
Israel DA, Salama N, Arnold CN, Moss SF, Ando T, Wirth HP, Tham KT, Camorlinga M, Blaser MJ, Falkow S, Peek RM Jr. (2001). Helicobacter pylori strain-specific differences in genetic content, identified by microarray, influence host inflammatory responses. J. Clin. Invest. 107: 611-620.
Crossref
 
Jaaskelainen AJ, Maunula L (2006). Applicability of microarray technique for the detection of noro- and astroviruses. J. Virol. Methods 136:210-216.
Crossref
 
Kakinuma K, Fukushima M, Kawaguchi R (2003). Detection and identification of Escherichia coli, Shigella, and Salmonella by microarrays using the gyrB gene. Biotechnol. Bioeng. 83:721-728.
Crossref
 
Kato-Maeda M, Rhee JT, Gingeras TR, Salamon H, Drenkow J, Smittipat N, Small PM (2001).Comparing genomes within the species Mycobacterium tuberculosis. Genome Res. 11:547-554.
Crossref
 
Khodakov DA, Zakharova NV, Gryadunov DA, Filatov FP, Zasedatelev AS, Mikhailovich VM (2008). An oligonucleotide microarray for multiplex real-time PCR identification of HIV-1, HBV, and HCV. BioTechniques 44:241-246, 248
Crossref
 
Koessler T, Francois P, Charbonnier Y, Huyghe A, Bento M, Dharan S, Renzi G, Lew D, Harbarth S, Pittet D, Schrenzel J (2006). Use of oligoarrays for characterization of community-onset methicillin-resistant Staphylococcus aureus. J. Clin. Microbiol. 44:1040-1048.
Crossref
 
Korczak B, Frey J, Schrenzel J, Pluschke G, Pfister R, Ehricht R, Kuhnert P (2005). Use of diagnostic microarrays for determination of virulence gene patterns of Escherichia coli K1, a major cause of neonatal meningitis. J. Clin. Microbiol. 43:1024-1031.
Crossref
 
Korimbocus J, Scaramozzino N, Lacroix B, Crance JM, Garin D, Vernet G (2005). DNA probe array for the simultaneous identification of herpesviruses, enteroviruses, and flaviviruses. J. Clin. Microbiol. 43:3779-3787.
Crossref
 
Kostic T, Weilharter A, Rubino S, Delogu G, Uzzau S, Rudi K, Sessitsch A, Bodrossy L (2007). A microbial diagnostic microarray technique for the sensitive detection and identification of pathogenic bacteria in a background of nonpathogens. Anal. Biochem. 360:244-254.
Crossref
 
Kozal MJ, Shah N, Shen N, Yang R, Fucini R, Merigan TC, Richman DD, Morris D, Hubbell E, M Chee, Gingeras TR (1996). Extensive polymorphisms observed in HIV-1 clade B protease gene using high-density oligonucleotide arrays. Nat. Med. 2:753-759.
Crossref
 
Lease RA, Belfort M (2000). A trans-acting RNA as a control switch in Escherichia coli: DsrA modulates function by forming alternative structures. Proc. Natl. Acad. Sci. USA 97:9919-9924.
Crossref
 
Lenhoff RJ, Naraghi-Arani P, Thissen JB, J Olivas, Carillo AC, Chinn C, Rasmussen M, Messenger SM, Suer LD, Smith SM, Tammero LF, Vitalis EA, Slezak TR, Hullinger PJ, Hindson BJ, Hietala SK, Crossley BM, McBride MT (2008). Multiplexed molecular assay for rapid exclusion of foot-and-mouth disease. J. Virol. Methods 153:61-69
Crossref
 
Li Y, Liu D, Cao B, Han W, Liu Y, Liu F, Guo X, Bastin DA, Feng L, Wang L (2006). Development of a serotype-specific DNA microarray for identification of some Shigella and pathogenic Escherichia coli strains. J. Clin. Microbiol. 44:4376-4383.
Crossref
 
Lopez MF, Pluskal MG (2003). Protein micro- and macroarrays: digitizing the proteome. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 787(1):19-27.
Crossref
 
Lovmar L, Fock C, Espinoza F, Bucardo F, Syvanen AC, Bondeson K (2003). Microarrays for genotyping human group A rotavirus by multiplex capture and type-specific primer extension. J. Clin. Microbiol. 41:5153-5158.
Crossref
 
MacBeath G (2002). Protein microarrays and proteomics. Nat. Genet. 32(Suppl.):526-532.
Crossref
 
Marlowe EM, Hogan JJ, Hindler JF, Andruszkiewicz I, Gordon P, Bruckner DA (2003). Application of an rRNA probe matrix for rapid identification of bacteria and fungi from routine blood cultures. J. Clin. Microbiol. 41:5127-5133.
Crossref
 
McLoughlin KS, (2011). Microarrays for Pathogen Detection and Analysis. Brief Funct Genomics.10(6):342-353.
Crossref
Mikhailovich V, Lapa S, Gryadunov D, Sobolev A, Strizhkov B, Chernyh N, Skotnikova O, Irtuganova O, Moroz A, Litvinov V, Vladimirskii M, Perelman M, Chernousova L, Erokhin V, Zasedatelev A, Mirzabekov A (2001). Identification of rifampin-resistant Mycobacterium tuberculosis strains by hybridization, PCR, and ligase detection reaction on oligonucleotide microchips. J. Clin. Microbiol. 39:2531-2540.
Crossref
 
Miller MB, Tang YW (2009). Basic Concepts of Microarrays and Potential Applications in Clinical Microbiology. Clin. Microbiol. Rev. 22(4):611-633.
Crossref
 
Monecke S, Ehricht R (2005). Rapid genotyping of methicillin-resistant Staphylococcus aureus (MRSA) isolates using miniaturised oligonucleotide arrays. Clin. Microbiol. Infect. 11:825-833.
Crossref
 
Monecke S, Berger-Bachi B, Coombs G, Holmes A, Kay I, Kearns A, Linde HJ, O'Brien F, Slickers P, Ehricht R (2007). Comparative genomics and DNA array- based genotyping of pandemic Staphylococcus aureus strains encoding Panton-valentine leukocidin. Clin. Microbiol. Infect. 13:236-249.
Crossref
 
Monecke S, Slickers P, Hotzel H, Richter-Huhn G, Pohle M, Weber S, Witte W, Ehricht R (2006). Microarray-based characterisation of a Panton-Valentine leukocidin-positive community-acquired strain of methicillin-resistant Staphylococcus aureus. Clin. Microbiol. Infect. 12:718-728.
Pubmed
 
Murakami S, Nakashima R, Yamashita E, and Yamaguchi A. (2002).Crystal structure of bacterial multidrug efflux transporter AcrB. Nature 419:587-593.
Crossref
 
Nsofor CA, Iroegbu CU, Davis MA, Orfe L, Call DR (2013). DNA microarray-based detection of antibiotic resistance genes of human isolates of Escherichia coli in Nigeria. J. Bacteriol. Res. 5(6):68-75
Crossref
 
Nubel U, Schmidt PM, Reiss E, Bier F, Beyer W, Naumann D (2004). Oligonucleotide microarray for identification of Bacillus anthracis based on intergenic transcribed spacers in ribosomal DNA. FEMS Microbiol. Lett. 240:215-223.
Crossref
 
Panicker G, Call DR, Krug MJ, Bej AK (2004). Detection of Pathogenic Vibrio spp. in shellfish by using multiplex PCR and DNA microarrays. Appl. Environ. Microbiol. 7436-7444
Crossref
 
Pas SD, Tran N, de Man RA, Burghoorn-Maas C, Vernet G, Niesters HG (2008). Comparison of reverse hybridization, microarray, and sequence analysis for genotyping hepatitis B virus. J. Clin. Microbiol. 46:1268-1273.
Crossref
 
Perreten V, Vorlet-Fawer L, Slickers P, Ehricht R, Kuhnert P, Frey J (2005). Microarray-based detection of 90 antibiotic resistance genes of gram-positive bacteria. J. Clin. Microbiol. 43:2291-2302.
Crossref
 
Peterson G, Jianfa B, Nagaraja TG, Sanjeev N (2010). Diagnostic microarray for human and animal bacterial diseases and their virulence and antimicrobial resistance genes. J. Microbiol. Methods 80:223-230
Crossref
 
Proudnikov D, Timofeev E, Mirzabekov A (1998). Immobilization of DNA in polyacrylamide gel for the manufacture of DNA and DNA-oligonucleotide microchips. Anal. Biochem. 259:34-41.
Crossref
 
Raddatz G, Dehio M, Meyer TF, Dehio C (2001). PrimeArray: genome-scale primer design for DNA microarray construction. Bioinformatics 17:98-99.
Crossref
 
Riehle MM, Bennett AF, Long AD (2001). Genetic architecture of thermal adaptation in Escherichia coli. Proc. Natl. Acad. Sci. USA 98:525-530.
Crossref
 
Roh SW, Guy CJ, Abell Kyoung-Ho Kim, Young-Do N and Jin-Woo B (2012). Comparing microarrays and nextgeneration sequencing technologies for microbial ecology research. Trends Biotechnol. 28 (6):234-256
 
Salama N, Guillemin K, McDaniel TK, Sherlock G, Tompkins L, Falkow S (2001). A whole-genome microarray reveals genetic diversity among Helicobacter pylori strains. Proc. Natl. Acad. Sci. USA 97:14668-14673.
Crossref
 
Scherl A, Francois P, Charbonnier Y, Deshusses JM, Koessler T, Huyghe A, Bento M, Stahl-Zeng J, Fischer A, Masselot A, Vaezzadeh A, Galle F, Renzoni A, Vaudaux P, Lew D, Zimmermann-Ivol CG, Binz PA, Sanchez JC, Hochstrasser DF, Schrenzel J (2006). Exploring glycopeptide-resistance in Staphylococcus aureus: a combined proteomics and transcriptomics approach for the identification of resistance-related markers. BMC Genomics 7:296.
Crossref
 
Selinger DW, Cheung KJ, Mei R, Johansson EM, Richmond CS, Blattner FR, Lockhart DJ, Church GM (2000). RNA expression analysis using a 30-base pair resolution Escherichia coli genome array. Nat. Biotechnol. 18:1262-1268.
Crossref
 
Shang S, Chen G, Wu Y, Du L, Zhao Z (2005). Rapid diagnosis of bacterial sepsis with PCR amplification and microarray hybridization in 16S rRNA gene. Pediatr. Res. 58:143-148
Crossref
 
Spiess B, Seifarth W, Hummel M, Frank O, Fabarius A, Zheng C, Morz H, Hehlmann R, Buchheidt D (2007). DNA microarray-based detection and identification of fungal pathogens in clinical samples from neutropenic patients. J. Clin. Microbiol. 45:3743-3753.
Crossref
 
Stover AG, Jeffery E, Xu JC, Persing DH (2003). Hybridization array, p. 619-639. In: D. H. Persing, F. C. Tenevor, J. Versalovic, Y. W. Tang, E. R. Unger, D. A. Relman, and T. J. White (ed.), Molecular microbiology: diagnostic principles and practice. ASM Press, Washington, DC.
 
Strizhkov BN, Drobyshev AL, Mikhailovich VM, Mirzabekov AD (2000). PCR amplification on a microarray of gel-immobilized oligonucleotides: detection of bacterial toxin- and drug-resistant genes and their mutations. BioTechniques 29:844-848, 850-852, 854.
Pubmed
 
Tang Y-W, Ellis NM, Hopkins MK, Smith DH, Dodge DE, Persing DH. (1998). Comparison of phenotypic and genotypic techniques for identification of unusual aerobic pathogenic Gram-negative bacilli. J. Clin. Microbiol. 36:3674-3679
Pubmed
 
Taniguchi M, Miura K, Iwao H, Yamanaka S (2001).Quantitative assessment of DNA microarrays—comparison with Northern blot analyses. Genomics 71:34-39.
Crossref
 
Troesch A, Nguyen H, Miyada CG, Desvarenne S, Gingeras TR, Kaplan PM, Cros P, Mabilat C (1999). Mycobacterium species identification and rifampin resistance testing with high-density DNA probe arrays. J. Clin. Microbiol. 37:49-55
Pubmed
 
Vahey M, Nau ME, Barrick S, Cooley JD, Sawyer R, Sleeker AA, Vickerman P, Bloor S, Larder B, Michael NL, Wegner SA (1999). Performance of the Affymetrix GeneChip HIV PRT 440 platform for antiretroviral drug resistance genotyping of human immunodeficiency virus type 1 clades and viral isolates with length polymorphisms. J. Clin. Microbiol. 37:2533-2537.
Pubmed
 
van Ijperen C, Kuhnert P, Frey J, Clewley JP (2002). Virulence typing of Escherichia coli using microarrays. Mol. Cell. Probes 16:371-378
Crossref
 
Volokhov D, Chizhikov V, Chumakov K, Rasooly A (2003). Microarray-based identification of thermophilic Campylobacter jejuni, C. coli, C. lari, and C. upsaliensis. J. Clin. Microbiol 41:4071-4080.
Crossref
 
Wang RF, Beggs ML, Erickson BD, Cerniglia CE (2004). DNA microarray analysis of predominant human intestinal bacteria in fecal samples. Mol. Cell. Probes 18:223-234
Crossref
 
Wang Z, Orlandi PA, Stenger DA (2005). Simultaneous detection of four human pathogenic microsporidian species from clinical samples by oligonucleotide microarray. J. Clin. Microbiol. 43:4121-4128
Crossref
 
Wapner RJ, Christa Lese Martin MD, Brynn Lev, Blake CB, Christine ME (2012). Chromosomal Microarray versus Karyotyping for Prenatal Diagnosis. N. Engl. J. Med. 367:2175-2184.
Crossref
 
Wei Y, Lee JM, Richmond C, Blattner FR, Rafalski JA, LaRossa RA (2001). High-density microarray-mediated gene expression profiling of Escherichia coli. J. Bacteriol. 183: 545-556.
Crossref
 
Willse A, Straub TM, Wunschel SC, Small JA, Call DR, Daly DS, Chandler DP (2004). Quantitative oligonucleotide microarray fingerprinting of Salmonella enterica isolates. Nucleic Acids Res. 32:1848-1856.
Crossref
 
Wilson M, DeRisi J, Kristensen HH, Imboden P, Rane S, Brown PO, Schoolnik GK (1999). Exploring drug-induced alterations in gene expression in Mycobacterium tuberculosis by microarray hybridization. Proc. Natl. Acad. Sci. USA 96: 12833-12838.
Crossref
 
Wilson JW, Bean P, Robins T, Graziano F, Persing DH (2000). Comparative evaluation of three human immunodeficiency virus genotyping systems: the HIV-GenotypR method, the HIV PRT GeneChip assay, and the HIV-1 RT line probe assay. J. Clin. Microbiol. 38:3022-3028.
Pubmed
 
Yang YH, Dudoit S, Luu P, Speed TP (2001). In: Normalization for cDNA Microarray Data. pp. 1-12
 
Ye RW, Wang T, Bedzyk L, Croker KM (2001). Applications of DNA microarrays in microbial systems. J. Microbiol. Methods 47:257-272.
Crossref
 
Ye Y, He X, Szewczyk P, Nguyen T, Chang G (2006). Structure of the multidrug transporter EmrD from Escherichia coli. Science 312:741-744.
Crossref
 
Ye RW, Tao W, Bedzyk L, Young T, Chen M, Li L (2000). Global gene expression profiles of Bacillus subtilis grown under anaerobic conditions. J. Bacteriol. 182: 4458-4465.
Crossref
 
You Y, Fu C, Zeng X, Fang D, Yan X, Sun B, Xiao D, Zhang J (2008). A novel DNA microarray for rapid diagnosis of enteropathogenic bacteria in stool specimens of patients with diarrhea. J. Microbiol. Methods 75:566-571.
Crossref
 
Yu H, David YW, Lee W, Siddaraju MN, Shivaprasad HV, Brian MB, Kamal DM (2013). Microarray Analysis Reveals the Molecular Basis of Antiarthritic Activity of Huo-Luo-Xiao-Ling Dan. Evid. Based Complement. Altern. Med 234-237.
 
Yue J, Shi W, Xie J, Li Y, Zeng E, Liang L, Wang H (2004). Detection of rifampin-resistant Mycobacterium tuberculosis strains by using a specialized oligonucleotide microarray. Diagn. Microbiol. Infect. Dis. 48:47-54
Crossref
 
Zhu LX, Zhang ZW, Wang C, Yang HW, Jiang D, Zhang Q, Mitchelson K, Cheng J (2007b). Use of a DNA microarray for simultaneous detection of antibiotic resistance genes among staphylococcal clinical isolates. J. Clin. Microbiol. 45:3514-3521.
Crossref
 
Zhu L-X, Zhang Z-W, Liang D, Jiang D, Wang C, Du N, Zhang Q, Mitchelson K, Cheng J (2007a). Multiplex asymmetric PCR-based oligonucleotide microarray for detection of drug resistance genes containing single mutations in Enterobacteriaceae. Antimicrob. Agents Chemother. 51:3707-3713.
Crossref