Staphylococcus aureus sequence type (ST) 1 isolated from sub clinical mastitis in settled Fulani herds in Kaduna State, Nigeria

Umaru G. A.; Kwaga J. K. P.; Bello M.; Raji M. A.; Joshua I. A.; Ido F. G.; Ba X. I.; Holmes M. A.

doi:10.5897/JMA2021.0446

Journal of
Microbiology and Antimicrobials

Abbreviation: J. Microbiol. Antimicrob.
Language: English
ISSN: 2141-2308
DOI: 10.5897/JMA
Start Year: 2009
Published Articles: 166

Full Length Research Paper

Staphylococcus aureus sequence type (ST) 1 isolated from sub clinical mastitis in settled Fulani herds in Kaduna State, Nigeria

Umaru G. A.

Umaru G. A.
Department of Veterinary Pathobiology, Faculty of Veterinary Medicine, Bayero University, Kano, Nigeria.
Search for this author on:
Google Scholar

Kwaga J. K. P.

Kwaga J. K. P.
Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria.
Search for this author on:
Google Scholar

Bello M.

Bello M.
Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria.
Search for this author on:
Google Scholar

Raji M. A.

Raji M. A.
Faculty of Veterinary Medicine, Department of Veterinary Microbiology, University of Ilorin, Kwara State, Nigeria.
Search for this author on:
Google Scholar

Joshua I. A.

Joshua I. A.
Department of Community Medicine, Faculty of Clinical Sciences, College of Medicine, Kaduna State University, Kaduna State, Nigeria.
Search for this author on:
Google Scholar

Ido F. G.

Ido F. G.
Avian Influenza Desk Office, Ministry of Agriculture and Natural Resources, Jalingo, Taraba State, Nigeria.
Search for this author on:
Google Scholar

Ba X. I.

Ba X. I.
Department of Clinical Veterinary Medicine, University of Cambridge, United Kingdom.
Search for this author on:
Google Scholar

Holmes M. A.

Holmes M. A.
Department of Clinical Veterinary Medicine, University of Cambridge, United Kingdom.
Search for this author on:
Google Scholar

Article Number - 9230EC271834
Vol.16(1), pp. 11-20 , January 2024
https://doi.org/10.5897/JMA2021.0446

Received: 23 June 2021
Accepted: 05 November 2021
Published: 29 February 2024

Abstract

A study was carried out to monitor the sequence type of Staphylococcus aureus from bovine mastitic milk in Kaduna State, Nigeria. The dairy cows and fresh milk samples were analyzed for clinical and subclinical mastitis, followed by isolation of S. aureus and antimicrobial susceptibility testing. Twenty-eight S. aureus were isolated phenotypically from bovine subclinical mastitis which were further examined molecularly for the acquisition of mec A, mec C and fem B genes. All the isolates were negative for mec A and mec C genes, while 3 isolates were positive for fem B gene. Typing using MLST revealed that the 3 fem B positive isolates were Sequence Type 1 (ST 1) and clonal complex 1, which had similar allelic profiles at all seven loci and showed high degree of monomorphism. The 3 MLST ST 1 isolates were recovered from cases of subclinical mastitis. The antibiotic susceptibility profile showed that the 3 MLST ST 1 isolates were multidrug resistant and resistant to commonly used antimicrobials. The phylogenetic relationship established using the nucleotide sequences revealed that the sequence of the strains MT550650, MT550651 and MT550652 had 99 % identity with the S. aureus BX571857, BA000033, AP015012, CP017115 and CP01780 available in the database. The MT550650, MT550651 and MT550652 S. aureus strains were found to be 100% homologous to each other with no divergence within the specie level. The isolation of ST 1 S. aureus strains from milk is of public health significance due to their role in food poisoning. Therefore, the study recommends proper hygienic procedures to reduce the contamination of milk by S. aureus and possible prevention of its spread to other animals and human population.