Abstract

The current study is skillful to detect the mutations of the H-RAS oncogene in patient of uterine cancer. Thirty specimens of blood and tissue were collected for DNA extraction, H-RAS oncogene amplification and histopathology examination. The results, which revealed presence of mutations in H-RAS oncogene represented by homozygous wild type Leu/Leu was 20%, mutant translocation genotype Val/Val was 48%, mutant deletion genotype Val/Leu was 22% and heterozygous genotype Leu /Val was 10%. The histopathological results of this study reveal too many types of uterine cancer. The major types of uterine cancer that appear in this study represented by endometrial adenosquamous carcinoma are  associated with endometrial lieomyosarcoma, the other  cases were represented as endometrial spheriodal cell carcinoma and as endometrial intra epithelial carcinoma.

Key words: H-RAS gene, uterine cancer, DNA.