April 2011

Glutathione-S-transferase (M1 and T1) polymorphisms in Nigerian populations

The Mu (μ) and Theta (Θ) classes of glutathione transferase gene, GSTM1 and GSTT1 are polymorphic in humans. GSTM1 and GSTT1 enzyme deficiencies have been suggested to predispose people to lung, bladder and colorectal cancer. This study was carried out to investigate the distribution of Glutathione transferases (GSTs) genotype frequency in the three major Nigerian ethnic groups with a view to understanding...

Author(s): Benjamin U. Ebeshi, Oluseye O. Bolaji and Collen M. Masimirembwa

April 2011

Evaluation of oxidative damage to peptic tissue DNA and gastric juice levels of nitric oxide and oxidative stress in smokers and non-smokers with signs of dyspepsia

Smoking elevates risk of peptic ulcer and gastrointestinal cancer in human being. In this study, the severity of the damage was assessed by detection of nitric oxide levels, oxidative stress of gastric juice in smokers and non-smokers. 43 smoker patients with active peptic ulcer as case group and 43 non-smokers without peptic ulcer, 43 smokers without peptic ulcer and 43 non-smokers with active peptic ulcer as control...

Author(s): Homayun Dolatkhah, Mohammad-Hasan Somi, Ebrahim Fattahi, Sahar Babaee, Mohammad Rahbani-Nobar, Manuchehr Nourazariyan and Behrooz Pourasghari

April 2011

Glutathione S- transferase M1 and T1 gene polymorphisms in South Indian stroke patients

Stroke is a multifactorial disease caused by the interaction of several genetic and environmental factors and a variety of risk factors such as blood pressure, diabetes, advanced age and smoking. Active cigarette smoking has been established as a major risk factor for coronary heart disease and for both ischemic and hemorrhagic stroke and the risk is particularly elevated in younger people. Glutathione S- transferases...

Author(s): Shehnaz Sultana, Venkata Kolla K., Vidyullatha P., Yasovanthi Jeedigunta, Pranay Penagaluru K., Sindhu Joshi, Usha Rani P. and Reddy P. P., 

April 2011

An alternative method for high throughput RNA isolation from whole blood

We have developed an efficient, cost effective, and high-throughput RNA isolation method for whole blood. By optimizing the blood processing steps and using the MagMAXÔ-96 platform from Applied BiosystemsÒ for high-throughput RNA isolation, we were able to isolate high quality and quantity RNA from whole blood using a simple and cost effective protocol that may be useful on a variety of blood...

Author(s): Damir Simic, Terry Van Vleet, Catherine Euler, Phillip Marx, R. Todd Bunch, W. Mike Peden and Thomas Sanderson