Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3832

Full Length Research Paper

Inhibition of nitric oxide production and mRNA expressions of proinflammatory mediators by xylarinic acid A in RAW 264.7 cells

Chang Geon Kim1, Tae-Wan Kim1, Mehari Endale1, Taddesse Yayeh1, Gon-Sik Seo2, Jae Yeul Cho3, Hwa-Jin Park4, Sang-Keun Kim5, In-Kyoung Lee6, Bong-Sik Yun6*and Man Hee Rhee1*
1Laboratory of Veterinary Physiology and Signaling, College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea. 2Department of Industrial Crops, Korea National College of Agriculture and Fisheries, Hwasung 445-760, Korea. 3School of Biotechnology and Bioengineering, Kangwon National University, Chuncheon 200-701, Korea. 4College of Biomedical Science and Engineering, and Regional Research Center, Inje University, Gimhae 621-749, 5College of Veterinary Medicine, Chungnam National University, Taejon 305-764, Korea. 6College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, Republic of Korea.
Email: [email protected], [email protected]

  •  Accepted: 02 October 2010
  •  Published: 18 November 2010

Abstract

Mushrooms possess vast and yet unknown source of powerful new pharmaceutical products, which contain active biomolecules. Xylarinic acid A isolated from a mushroom,Xylaria polymorpha, has been reported to have free radicals scavenging property and anti-fungal activity. However, information of its anti-inflammatory activity is scarce. Therefore, the objective of this study was to examine the effect of the compound on the gene expression levels of inflammatory mediators and pro-inflammatory cytokines. MTT assay, NO assay, semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and quantitative real time PCR (qPCR) were performed to determine the effect of xylarinic acid on cell viability, NO production and mRNA expression levels in LPS-treated RAW 264.7 mouse macrophage-like cells. As such, xylarinic acid A inhibited nitric oxide production and mRNA expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-1b (IL-1b) and interleukin-6 (IL-6) in a concentration dependant pattern without cytotoxicity effect. Our preliminary result indicates that xylarinic acid A might have anti-inflammatory property.

 

Key words: Xylarinic acid A, macrophages, inflammation, inflammatory mediators.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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