Abstract

This study was done to evaluate the amount of phytochemicals (phenols and flavonoids) content, antioxidant and antibacterial characteristics of the various extracts of the fruit ofRosa canina from Iran through various in vitro methods. Among the extracts, the methanol fraction with 11.58  µg/ml and 88.7% antioxidant  activities  in  terms  of  IC50  values using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), respectively, was the most powerful ones.  A study was done on the relationship between antioxidant capacity and total phenolic and flavonoid content, indicating that extracts containing these compounds are the major contributors to the antioxidant properties. According to the antibacterial activity of the various extracts, the methanol fraction was the most impressive extract against the available microorganism. Based on the strong antioxidant and antibacterial activities, the methanol fraction of R. canina fruit appears to be a potential herb and can be further explored as a functional medicinal plant for isolating the active ingredient(s), especially among flavonoids, along with animal studiesin vivo.

Key words: Antibacterial, antioxidant, Rosa canina, total flavonoid content, total phenolic content.