Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Isolation and structure determination of a new oligosaccharide from Blume riparia

Zijing Xu1, Cuiwu Lin1* and Yong Shi2
1College of Chemistry and Chemical Engineering, Guangxi University, Nanning Guangxi 530004, China. 2Guangxi Medicinal Plants Botantical Garden, Nanning Guangxi 530023, China.
Email: [email protected]

  •  Accepted: 10 May 2011
  •  Published: 18 July 2011

Abstract

A water-soluble oligosaccharide (BROS) was obtained from Blume riparia DC. by hot water extraction, ethanol precipitation, and fractionated by Sephadex G-50 and Sephadex G-25 column filtration chromatography. BROS is a homogenous composition. Its molecular weight was 1.314×103 Da by MALDI-TOF-MS accurate determination. It was composed of 8 monosaccharides, namely Glcp, Frup and Fruf in molar ratio of 1:1:6. The linkage type of all residues were determined by the means of methylation. A combination of 1H NMR and13C NMR spectroscopy analysis, the BROS structure was confirmed finally as: β-D-Frup-(2→1)-β-D-Fruf-1-(2→1)-β-D-Fruf-2-(2→1)-β-D-Fruf-3-(2→1)-β-D -Fruf-4-(2→1)-β-D-Fruf-5-(2→1)-β-D-Fruf-6-(2→1)-α-D-Glcp.

 

Key words: Blumea riparia (Blume) DC, oligosaccharide (BROS), purification, methylation, spectroscopy, structure.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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