Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3837

Full Length Research Paper

Optimization of two-dimensional gel electrophoresis protocols for Boesenbergia rotunda in vitro suspension culture

Tan Eng Chong1,4*, Foo Gen Teck3,5, Wong Sher Ming3,5, Noorsaadah Abd Rahman2,4, Norzulaani Khalid3,5, Saiful Anuar Karsani3,4, Shatrah Othman1,4 and Rohana Yusof1,4
1Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. 2Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. 3Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. 4Drug Design and Development Research Group, University of Malaya, 50603 Kuala Lumpur, Malaysia. 5Biotechnology and Bioproduct Research Cluster (UMBIO), University of Malaya, 50603 Kuala Lumpur, Malaysia.
Email: [email protected], [email protected], [email protected]

  •  Accepted: 19 May 2011
  •  Published: 18 August 2011

Abstract

Boesenbergia rotunda belongs to the Zingiberaceae family and is abundant in the Southeast Asia. It is widely used as food ingredient and traditional medicine. Biologically, the plant extract contains pharmaceutically important bioactive compounds that exhibit anti-HIV protease, antibacterial, antifungal, anti-inflammatory, anti-tumor and antioxidant activities. Proteomics approaches to study the proteins and/or enzymes involved in the biosynthesis of these compounds are challenging due to the complexity of plant samples and the presence of interfering substances. Here, we describe the development a highly robust and reproducible two-dimensional gel electrophoresis (2DE) protocols for resolving the proteome of B. rotunda suspension cultures.

 

Key words: Two dimensional electrophoresis, plant proteomics, Boesenbergia rotunda, Temu kunci, callus.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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