Abstract

Rosa canina is a medicinal plant that its fruits have many important medicinal properties but unknown for many people's especially in Iran. However, this crop can be produced commercially and its orchards can be established such as other fruit trees. This research was carried out for studing pollen germination and tube growth as main factors affecting fruit set, in some Hashtroud and Maragheh indigenous genotypes of R. canina. Experiment was carried out based on completely randomized design (CRD) in ten genotypes (treatments) with four repeats. Pollens were cultured in an in-vitro medium containing 15% Sucrose, 0.01% Boric Acid and 1.2%. Agar and then maintained in 24°C in controlled condition. After 24 h pollens germination and growth were stopped with cholorophorm. Finally, pollen germination percentage (PGP) and pollen tube length (PTL) were measured by means of Light-Microscope at least in 7 randomized selected squares in each Petri Dish (repeats). Data analyzed with SAS software and results showed significant differences between studied characteristics of genotypes.

Key words: Rosa canina, pollen germination, pollen tube growth, in vitro.