Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3832

Full Length Research Paper

Development of phytochemical fingerprint of an Indian medicinal plant Chitrak (Plumbago zeylanica L) using high performance thin layer chromatography (HPTLC)

I. C. Mohanty*
  • I. C. Mohanty*
  • Department of Agricultural Biotechnology, College of Agriculture, Orissa University of Agriculture and Technology, Bhubaneswar-751003, Orissa, India.
  • Search for this author on:
  • Google Scholar
Vishnu K. Soni
  • Vishnu K. Soni
  • Department of Agricultural Biotechnology, College of Agriculture, Orissa University of Agriculture and Technology, Bhubaneswar-751003, Orissa, India.
  • Search for this author on:
  • Google Scholar
G. R. Sahu
  • G. R. Sahu
  • Department of Agricultural Biotechnology, College of Agriculture, Orissa University of Agriculture and Technology, Bhubaneswar-751003, Orissa, India.
  • Search for this author on:
  • Google Scholar


  •  Received: 21 August 2011
  •  Accepted: 15 April 2014
  •  Published: 10 May 2014

Abstract

Fresh, shade-dried and powdered samples of leaf, stem and root of Chitrak (Plumbago zeylanica L) were subjected to fractional distillation in a Soxhlet apparatus using four organic solvents. Chloroform, acetone and ethanol extracts from root of Plumbago showed higher zone of inhibition of 22.66±1.52, 21.5±1.29 and 16.5±1.29 mm and minimum inhibitory concentration (MIC) of 1.0, 10.0 and 10.0 μg/ml, respectively against Escherichia coli. Root and leaf extracts by ethanol, chloroform and acetone showed higher antibacterial activity against E. coli as compared to standard Kanamycin (MIC-100 μg/ml). The high performance thin layer chromatography (HPTLC) fingerprints were used for the quantitation of two bioactive markers: gibberellic acid and quinol R in the plant powder of different organs. Maximum content of gibberellic acid was found in acetone extract of the root (59.74%, Rf 0.79) followed by methanol root extract (53.01%). HPTLC provides a chromatographic fingerprint of phytochemicals and is suitable for confirming the identity and purity of medicinal plant raw materials.

Key words: Plumbago zeylanica, antimicrobial property, bioactive compound, kanamycin, Escherichia coli, high performance thin layer chromatography (HPTLC), phytochemical fingerprint.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 8 No. 18
Back to articles
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES