Abstract

The methanolic extract of the stems of Montanoa bipinnatifida was investigated for its phenolic compounds and hepactoprotective activity. A new flavon C-glycosides named as luteolin 6-C- apioside 8-C- glucoside (1) as well as apigenin 6-C-glucoside(2), apigenin 8-C-glucoside(3), apigenin 6,8- di-C-glucoside(4), luteolin 8-C-glucoside(5) luteolin 6,8- di-C-glucoside(6), luteolin 6-C- glucoside(7), apigenin(8) and luteolin(9) were isolated from the M. bipinnatifida stems aqueous alcoholic extract. Structures of the isolated compounds were established by chromatography, UV and 1D∕2D ¹H∕ ¹³C spectroscopy. Hepatoprotective and antioxidant effects were investigated for the aqueous methanol extract of M. bipinnatifida stems (100 and 150mg/Kg b.wt.) in normal and carbon tetrachloride –hepatic damaged rats. The hepatotoxic dose of CCl₄ (25 mg/Kg, orally) raised significantly (P< 0.05) the serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels as compared to control values. The dose aqueous methanolic extract of M. bipinnatifida stems were able to prevent CCl₄-induced rise in serum enzymes.

Key words: Montanoa bipinnatifida, flavone-C-glycosides, CCl₄, hepatoprotective.