Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Improved in vitro plant regeneration and micro propagation of Rehmannia glutinosa L.

Sang Un Park1, Yong Kyoung Kim1 and Sook Young Lee2*
1Division of Plant Science and Resources, Chungnam National University, 220Gung-Dong, Yuseong-Gu, Daejeon, 305-764, Korea. 2Research Center for Proteineous Materials, Chosun University, 375 Seosuk-Dong, Dong-Gu, Gwangju, 501-759, Korea.  
Email: [email protected]

  •  Accepted: 04 December 2008
  •  Published: 31 January 2009

Abstract

The establishment of an efficient protocol for plant regeneration and micropropagation from leaf cultures of Rehmannia glutinosa L. is reported. The regenerated shoots obtained from leaf cultures on solid MS medium containing different concentrations of TDZ. The highest number of shoots per explant (2.1) and shoot growth (1.2 cm) was obtained on MS medium containing 1 mg/L TDZ. The addition of 0.1 mg/L NAA and3g/L Gelrite in MS medium containing 1 mg/L TDZ substantially improved the shoot regeneration of R. glutinosa. The rooted plants were hardened and transferred to soil with a 73% survival rate. The continuous production of R. glutinosa regenerated plants could be used as a possible micropropagation system.

 

Key words: Micropropagation, plant regeneration, shoot organogenesis,Rehmannia glutinosa L.

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