Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Comparison of genetic diversity in Pogostemon cablin from China revealed by RAPD, morphological and chemical analyses

Lianhua Wu1,3, Yougen Wu1*, Qiaosheng Guo2, Shaopeng Li1, Kaibing Zhou1 and Junfeng Zhang4
1Key Laboratory of Protection, Development and Utilization of Tropical Crop Germplasm Resources (Hainan University) of Ministry of Education, College of Horticulture and Landscape, Hainan University, Haikou 570228, P. R. China. 2Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, P. R. China. 3Department of Pharmacy, Guiyang College of Traditional Chinese Medicine, Guiyang 550002, P. R. China. 4Material and Chemical Engineering College, Hainan University, Haikou 570228, P. R. China
Email: [email protected]

  •  Accepted: 29 July 2011
  •  Published: 16 September 2011

Abstract

The genetic diversity of 16 patchouli populations by random amplified polymorphic DNA (RAPD), morphology and chemical composition was presented in this paper. Polymorphism of RAPD primers and the percentage of polymorphic bands revealed with RAPD were 79.21 and 78.40%, respectively, which indicated a high level of genetic diversity existed among Pogostemon cablin (Blanco) populations. A dendrogram based on RAPD analysis showed most populations from the same or adjacent regions were classified together. High polymorphism in morphological parameters was found among populations, but did not reflect completely the differences by geographic location. According to the relative contents of nine compounds in P. cablin, the tested samples were divided into two main clusters, pogostone-type and patchouliol-type. A correlation between genetic and chemical polymorphism (r2 = 0.816) was higher than that between genetic and morphological variations (r2 = 0.629). High morphological and chemical variability as well as genotypic polymorphism provided ecological advantages that might explain the extensive distribution of P. cablin.

 

Key words: Pogostemon cablin (Blanco) Benth, genetic diversity, random amplified polymorphic DNA (RAPD), morphology, essential oil.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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