Abstract

In this study was performed chemical analysis on alcoholic extracts of the Scilla bifolia (Liliaceae family) species  harvested from spontaneous flora, with the purpose of determining bioactive cardiac glycosides, particularly proscillaridin A. The used analysis techniques were thin layer chromatography (TLC) and high performance liquid chromatography coupled with mass spectrometry (HPLC-MS). After performing TLC analysis, the presence of the scillarosides was confirmed, but only in traces, in higher amounts in herba than in bulbs. The presence of proscillaridin A has not been determined in any analysed samples, either before or after hydrolysis by HPLC-MS. Thus, we can conclude that, the bulbs and aerial parts of Scilla bifolia do not contain proscillardin A.

Key words: HPLC-MS analysis, alcoholic extract, proscillaridin A, Scilla bifolia, TLC analysis.