Abstract

Warburgia ugandensis is an important medicinal tree species whose bark is widely harvested for its valuable anti-bacterial and anti-fungal properties. Consequently, this tree species is considered threatened. Therefore, this species is ranked as one of the priority species for management and conservation. This study investigated an appropriate technique for propagation of W. ugandensis using stem cuttings. Three types of stem cutting (hardwood, semi-hardwood and softwood) were obtained from Mabira Forest Reserve, Uganda and propagated in a factorial experiment using non-misting tunnels. Data on callusing root and shoot formation, number and length of roots and shoots were collected over a period of 93 days. Data manipulation was done by employing general linear model analysis of variance and Chi - square tests.  There was significant variation (p < 0.05) in callus formation, root and shoot development, number and length of roots and shoots for different stem cutting types. The highest percentage of callusing, rooting and shoot regeneration (46, 49 and 57%) was recorded in softwood cuttings which also produced the highest number and longest roots and shoots. Successful propagation of W. ugandensis can therefore be appropriately achieved through softwood stem cuttings rather than either hardwood or semi-hardwood cuttings.

Key words: Warburgia ugandensis, vegetative propagation, Mabira forest reserve, Canellaceae.