Abstract

Antimicrobial activity of five compounds isolated from Ranunculus muricatus, namely, Tricin7-O-β-D-lucopyranoside (1), protocatechuyl aldehide (2), isoscopoletin (3), anemonin (4), Β-sitoseterol (5) were tested against two human Gram-positive bacteria (Staphylococcus aureus, Micrococcus luteus) and four Gram-negative ones (Escherichia coli, Pseudomonas aeruginosa, Enterobacter cloacae, Klebsiella Pneumoniae). All the five compounds isolated, were tested against the fungal strain Aspergilus niger. The structures of the compounds have been confirmed by EIS, HR-EIMS and ¹H-¹³C-NMR spectroscopic methods. The antimicrobial activities were performed by agar well diffusion method. MIC and MBC were carried out by agar dilution method and viable cell count method, respectively. Compound 1 showed maximum antimicrobial activities while the other compounds also showed superior antimicrobial activities. These compounds were isolated first time from R. muricatus which are persuasive as standard antimicrobial drugs.

Key words: Ranunculus muricatus, isolation, antimicrobial activity, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC).