Abstract

The present research work was carried out to investigate the antimicrobial (8 bacteria and 1 fungus) activities of different solvent (petroleum ether, ethyl acetate, chloroform, iso-butanol and ethanol) extracted samples from leaves of Plumeria obtusa by disc diffusion method. The data revealed that petroleum ether, iso-butanol and ethyl acetate fractions showed inhibitory activities against all the nine microbial species except Klebsiella pneumonia and Pseudomonas aeruginosa, respectively. The most susceptible gram-positive bacterium was Bacillus subtilis while the most resistant gram-positive bacterium was Staphylococcus aureus. Erwinia carotovora was the most susceptible gram-negative bacterium while P.aeruginosa was highly resistant among the gram- negative bacteria.

Key words: Antimicrobial activity, solvents, fungus, disc diffusion, Plumeria obtusa.