Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Extraction and analysis of β-sitosterol in herbal medicines

Je-Chiuan Ye1, Wei-Chun Chang 2, Dennis Jine-Yuan Hsieh3, Meen-Woon Hsiao*4
1Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan. 2Department of Obstetrics and Gynecology, China Medical University Hospital, Taichung, Taiwan. 3Department of Medical Technology, Chung Shan Medical University, Taichung, Taiwan. 4School of Applied Chemistry, Chung Shan Medical University, Department of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan
Email: [email protected]

  •  Accepted: 25 February 2010
  •  Published: 04 April 2010

Abstract

Knoxia valerianoides is a herbal medicine and β-sitosterol is one of its main components. β-sitosterol is known to control cholesterol levels, reduce the activity of cancer cell, promote prostate gland health and enhance immunity in the human body. β-sitosterol can also be found in vegetable oils such as: wheat germ oil, cotton seed oil and so on. The amounts of β-sitosterol in herbal medicines and vegetable oils have not been reported in the literature since an analytical method has not yet been well established. This paper shows that high performance liquid chromatography (HPLC) is a suitable analytical method for determiing β-sitosterol levels in Knoxia valerianoids Thorel and pitard (Hun Da Ji), Jing Da Ji and several kinds of vegetable oils.

 

Key words: β-sitosterol, Knoxia valerianoids, Soxhlet extraction and HPLC

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