Abstract

The effective composition content were determined in Cultivated and wild of Epimedium pubescens and Epimedium wushanense by High-Pressure Liquid Chromatography (HPLC) and ultraviolet-visible (UV), and the difference of morphological parameters and effective composition content were analyzed by one-way ANOVA statistical analysis. The results showed that because of the relative light intensity increased, the dwarf phenomenon was occurred in Cultivated E. pubescens and E. wushanense, but the number of branches and leaf number per plant in cultivated were more than wild, and increased the output of two kinds of Epimedium. According to the standard of Chinese Pharmacopoeia, both wild and cultivated leaves the icariin contents of E. pubescens can meet the standard. Insignificant difference in flavonoid contents between the cultivated and wild leaves of E. wushanense, but both can meet the standard, both wild and cultivated all other parts of E. wushanense cannot meet the standard of icariin contents, which may be related to harvesting time.

Key words: Epimedium pubescens, Epimedium wushanense, wild and cultivated, effective composition, morphological characteristics.