Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Micormorphological diversity in leaf epidermal anatomy of Brachiaria species using elemental dispersive spectrophotometer analysis

Shabnum Shaheen1, Mushtaq Ahmad2, Farah Khan1, Muhammad Zafar2*, Mir Ajab Khan2, Asghari Bano2, Shazia Sultana2, Muhammad Jamil3, Gul Jan4 and Muhammad Khan Leghari5
1Department of Botany, Lahore College for Women University Lahore, Pakistan. 2Department of Plant Sciences, Quaid-i-Azam University Islamabad, Pakistan. 3Department of Biotechnology and Genetic Engineering, Kohat University of Science of Technology (KUST) Kohat, Pakistan. 4Department of Botany, Hazara University, Mansehra, Pakistan. 5Pakistan Science Foundation, Ministry of Science and Technology, Islamabad, Pakistan.
Email: [email protected], [email protected]

  •  Accepted: 09 June 2011
  •  Published: 09 September 2011

Abstract

In this study the micromorphological diversity linked to different elements silica bodies, epidermal leaf anatomy and elemental dispersive spectrophotometer (EDS) analysis (LM, SEM) of five species of genus Brachiaria was carried out. The technique of EDS analysis for grass taxonomy is used first time in Pakistan. This examination indicates variations in leaf blade epidermal characters such as shapes of silica bodies, prickles, and microhair and macrohair form. EDS analysis of the phytoliths determines a new structure used as an aid in phytosystematic characterization. The quantitative analysis provides silicon as a new taxonomic character to distinguish different species of the genus Brachiaria.

 

Key words: Epidermal anatomy, micropalynological diversity, elemental dispersive spectrophotometer.